Rapid Method for the Radioisotopic Analysis of Gaseous End Products of Anaerobic Metabolism
| Autor: | David R. Nelson, J. G. Zeikus |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Chromatography
Gas Formates Hydrogen Sarcina ved/biology.organism_classification_rank.species chemistry.chemical_element Proportional counter Fresh Water General Biochemistry Genetics and Molecular Biology Methane chemistry.chemical_compound Methods Organic matter Anaerobiosis Carbon Radioisotopes General Pharmacology Toxicology and Pharmaceutics Cellulose Soil Microbiology Environmental Microbiology and Ecology chemistry.chemical_classification Chromatography General Immunology and Microbiology ved/biology General Medicine Carbon Dioxide Decomposition chemistry Evaluation Studies as Topic Carbon dioxide Methanosarcina barkeri Gases Gas chromatography Water Microbiology |
| Zdroj: | Applied Microbiology. 28:258-261 |
| ISSN: | 0003-6919 |
| Popis: | A gas chromatographic procedure for the simultaneous analysis of 14 C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described. H 2 , CH 4 , and CO 2 were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity. Detector effluents were channeled into a gas proportional counter for measurement of radioactivity. This method was more rapid, sensitive, and convenient than gas chromatography-liquid scintillation techniques. The gas chromatography-gas proportional counting procedure was used to characterize the microbial decomposition of organic matter in anaerobic lake sediments and to monitor 14 CH 4 formation from H 2 and 14 CO 2 by Methanosarcina barkeri . |
| Databáze: | OpenAIRE |
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