Temperature and nutrient availability control growth rate and fatty acid composition of facultatively psychrophilic Cobetia marina strain L-2
| Autor: | Kikue Hirota, Yoshio Ezura, Keita Kusumoto, Hidetoshi Okuyama, Hidetoshi Matsuyama, Masatoshi Akutsu, Hideaki Iwata, Isao Yumoto, Naoki Morita |
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| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Strain (chemistry) Cobetia marina Cell Membrane Fatty Acids Temperature Fatty acid General Medicine Bacterial growth Biology biology.organism_classification Biochemistry Microbiology Culture Media Cold Temperature Halomonadaceae Chemically defined medium chemistry Fatty Acids Unsaturated Genetics Yeast extract Growth rate Psychrophile Molecular Biology |
| Zdroj: | Archives of Microbiology. 181:345-351 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-004-0662-8 |
| Popis: | A facultative psychrophilic bacterium, strain L-2, that grows at 0 and 5 degrees C as minimum growth temperatures in complex and defined media, respectively, was isolated. On the basis of taxonomic studies, strain L-2 was identified as Cobetia marina. The adaptability of strain L-2 to cold temperature was higher than that of the type strain and of other reported strains of the same species. When the bacterium was grown at 5-15 degrees C in a defined medium, it produced a high amount of trans-unsaturated fatty acids. By contrast, in a complex medium in the same temperature range it produced a low amount of trans-unsaturated fatty acids. In the complex medium at 5 degrees C, the bacterium exhibited a three-fold higher growth rate than that obtained in the defined medium. Following a temperature shift from 11 to 5 degrees C, strain L-2 grew better in complex than in defined medium. Furthermore, when the growth temperature was shifted from 0 to 5 degrees C both the growth rate and the yield of strain L-2 growing in complex medium was markedly enhanced. These phenomena suggest that an upshift of the growth temperature had a positive effect on metabolism. The effects of adding complex medium components to the defined medium on bacterial growth rate and fatty acid composition at 5 degrees C were also studied. The addition of yeast extract followed by peptone was effective in promoting rapid growth, while glutamate addition was less effective, resulting in a cis-unsaturated fatty acid ratio similar to that of cells grown in the complex medium. These results suggest that the rapid growth of strain L-2 at low temperatures requires a high content of various amino acids rather than the presence of a high ratio of cis-unsaturated fatty acids in the cell membrane. |
| Databáze: | OpenAIRE |
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