Transmission infrared spectroscopy of whole blood--complications for quantitative analysis from leucocyte adhesion during continuous monitoring
| Autor: | Uwe Damm, Mathias D. Brendel, Steffen Leonhardt, Venkata Radhakrishna Kondepati, Bayden R. Wood, H. Michael Heise, Thorsten Vahlsing |
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| Rok vydání: | 2010 |
| Předmět: |
Spectrophotometry
Infrared Chemistry Swine Continuous monitoring General Engineering Analytical chemistry General Physics and Astronomy Infrared spectroscopy General Chemistry Blood flow General Biochemistry Genetics and Molecular Biology Cellular material Cell Adhesion Leukocytes Animals Humans General Materials Science Sample path Artifacts Quantitative analysis (chemistry) Cells Cultured Leucocyte adhesion Biomedical engineering Whole blood |
| Zdroj: | Journal of biophotonics. 3(8-9) |
| ISSN: | 1864-0648 |
| Popis: | Infrared spectroscopy has been applied to analyse glucose and cellular components in whole blood with the aim of developing an online clinical diagnostic and monitoring modality. Leucocyte adsorption onto the CaF2 windows was observed over a period of several hours under continuous blood flow using a transmission cell of 30 μm path length. This build-up of cellular material on the windows is responsible for diminishing the sample path length under the flow conditions chosen. The adsorption dynamics have been characterised and their impact on glucose monitoring is reported. For short-term monitoring ( |
| Databáze: | OpenAIRE |
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