Identification of grapevines infected with divergent variants of Grapevine virus A using variant-specific RT-PCR
| Autor: | A.E.C. Jooste, D.E. Goszczynski |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Genetics
Cloning biology Base Sequence Specific detection Reverse Transcriptase Polymerase Chain Reaction viruses fungi Molecular Sequence Data Nicotiana benthamiana Genetic Variation Single-strand conformation polymorphism biology.organism_classification Virology Virus Plant Viruses Reverse transcription polymerase chain reaction Rapid identification Real-time polymerase chain reaction DNA Viral Vitis |
| Zdroj: | Journal of virological methods. 112(1-2) |
| ISSN: | 0166-0934 |
| Popis: | RT-PCRs, designed for the specific detection of molecular variants of Grapevine virus A (GVA), were applied to analysis of the virus from various grapevines and isolates recovered from these grapevines in Nicotiana benthamiana. Results of SSCP, cloning and sequencing revealed that the combination of these RT-PCR techniques permits reliable and rapid identification of grapevines mixed-infected with divergent variants of GVA. The results suggest that such grapevines are very common among GVA-infected grapevines in vineyards in South Africa. |
| Databáze: | OpenAIRE |
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