A medically relevant capsular polysaccharide in Acinetobacter baumannii is a potential vaccine candidate
| Autor: | Shui-Tsung Chen, Yi-Tzu Lee, Jeffy Chern, Wan-Ling Wu, Nien-Tsung Lin, Shu-Chen Kuo, Wei Zou, Shih-Hsiung Wu, Feng-Ling Yang, Tze-Chi Lou |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Acinetobacter baumannii medicine.medical_treatment Taiwan Passive immunity Polysaccharide Microbiology Mice 03 medical and health sciences Animal model medicine Animals Humans clinic population distribution Longitudinal Studies Gel electrophoresis chemistry.chemical_classification General Veterinary General Immunology and Microbiology biology Polysaccharides Bacterial Public Health Environmental and Occupational Health biology.organism_classification Virology capsular polysaccharide Disease Models Animal Cross-Sectional Studies 030104 developmental biology Infectious Diseases chemistry passive immunity Bacterial Vaccines biology.protein Molecular Medicine bacteria Antibody Bacterial outer membrane Acinetobacter Infections |
| Popis: | Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection. |
| Databáze: | OpenAIRE |
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