Protein zero of peripheral nerve myelin: biosynthesis, membrane insertion, and evidence for homotypic interaction
| Autor: | D. D’Urso, David R. Colman, Alan B. Frey, Peter J. Brophy, C. Stewart Gillespie, Susan M. Staugaitis, Jerome G. Stempak |
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| Rok vydání: | 1990 |
| Předmět: |
Lipid Bilayers
Molecular Sequence Data Fluorescent Antibody Technique Endoplasmic Reticulum Transfection Myelin Complementary DNA medicine Extracellular Animals Amino Acid Sequence RNA Messenger Myelin Sheath chemistry.chemical_classification Membrane Glycoproteins biology General Neuroscience Myelin protein zero Cell Membrane Rats Inbred Strains Sciatic Nerve Cell biology Rats Molecular Weight Membrane glycoproteins Microscopy Electron medicine.anatomical_structure Biochemistry chemistry Protein Biosynthesis biology.protein RNA Electrophoresis Polyacrylamide Gel Glycoprotein Peptides Poly A Myelin P0 Protein Intracellular Myelin Proteins HeLa Cells Plasmids |
| Zdroj: | Neuron. 4(3) |
| ISSN: | 0896-6273 |
| Popis: | Protein zero (P 0 ), an integral membrane glycoprotein synthesized by Schwann cells, is the major glycoprotein of peripheral nerve myelin. The predicted disposition of P 0 with respect to the membrane bilayer postulates the existence of extracellular and intracellular domains, that mediate compaction of the myelin lamellae. We used in vitro translations programed with sciatic nerve mRNA and cells transfected with a P 0 cDNA construct to study the biosynthesis and topology of P 0 in the bilayer. The behavior of P 0 at the cell surface, when expressed under physiological conditions, was also examined. We have verified the topological predictions of an earlier model, derived from analysis of a P 0 cDNA, and provide evidence that the extracellular domain of P 0 mediates homotypically cell-cell interactions in the transfectants. |
| Databáze: | OpenAIRE |
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