Phosphate as a Limiting Factor for the Cell Division of Tobacco BY-2 Cells
| Autor: | Yoshiki Kuraya, Shin-ichi Amino, Toshiyuki Nagata, Toshio Sano |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Tobacco BY-2 cells
Cytoplasm DNA Complementary Cell division Physiology ATPase Molecular Sequence Data Fluorescent Antibody Technique Plant Science Biology Genes Plant Phosphates Gene product Cell membrane Gene Expression Regulation Plant Tobacco medicine Amino Acid Sequence Cells Cultured Plant Proteins Sequence Homology Amino Acid DNA synthesis Cell Membrane Membrane Proteins Cell Biology General Medicine Cell cycle Cell biology Plants Toxic Proton-Translocating ATPases medicine.anatomical_structure Biochemistry biology.protein Cell Division |
| Zdroj: | Plant and Cell Physiology. 40:1-16 |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | The re-addition of phosphate to tobacco BY-2 cells deprived of phosphate for 3 d induced cells to semi-synchronously re-enter the cell cycle from a static state. Though the addition of auxin to auxin-starved tobacco BY-2 cells also induced cell division (Ishida et al. 1993), some major differences were observed between these two systems. BY-2 cells lost the ability to re-enter the cell cycle after prolonged periods of auxin deprivation, but in contrast retained this ability after longer periods in the absence of phosphate. By differential cDNA screening we identified a phosphate-induced gene phi-1. phi-1 was rapidly induced by the addition of phosphate with transcript levels starting to decrease by the start of DNA synthesis. phi-1 does not share any significant homology with any gene with known functions over its full length. However, the N-terminus shared some homology with plasma membrane ATPases suggesting that it may be involved in some process of phosphorylation. Immunolocalization of the phi-1 gene product revealed that it rapidly accumulated in the cytoplasm prior to the start of plastid and nuclear DNA synthesis. These results are discussed in relation to the role of phosphate in inducing plant cell division. |
| Databáze: | OpenAIRE |
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