mRNA-Selective Translation Induced by FSH in Primary Sertoli Cells
| Autor: | Julia Morales, Nathalie Oulhen, Astrid Musnier, Patrick Vourc'h, Sandrine Boulben, Kelly León, Domitille Heitzler, Eric Reiter, Thomas Boulo, Anne Poupon, Patrick Cormier, Pascale Crépieux, Vlad Costache |
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| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Mer et santé (MS), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université européenne de Bretagne - European University of Brittany (UEB), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biochimie et de Biologie Moléculaire, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Imagerie et cerveau, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Eukaryotic Initiation Factor-4E 0302 clinical medicine Endocrinology Eukaryotic initiation factor Protein biosynthesis Phosphorylation Cells Cultured Original Research 0303 health sciences TOR Serine-Threonine Kinases Intracellular Signaling Peptides and Proteins Ribosomal Protein S6 Kinases 70-kDa General Medicine Sertoli cell Cell biology medicine.anatomical_structure fsh Receptors FSH Signal transduction Proto-Oncogene Proteins c-fos RNA Caps medicine.medical_specialty sertoli cell endocrine system Primary Cell Culture Biology 03 medical and health sciences Internal medicine Polysome medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Rats Wistar Molecular Biology 030304 developmental biology Messenger RNA Sertoli Cells Phosphoproteins Rats réseau de signalisation Polyribosomes Protein Biosynthesis cellule de sertoli RAT Follicle Stimulating Hormone Carrier Proteins Eukaryotic Initiation Factor-4G 030217 neurology & neurosurgery |
| Zdroj: | Molecular Endocrinology-Baltimore Molecular Endocrinology-Baltimore-, 2012, 26 (4), pp.669-680. ⟨10.1210/me.2011-1267⟩ Molecular Endocrinology-Baltimore-, Endocrine Society, 2012, 26 (4), pp.669-680. ⟨10.1210/me.2011-1267⟩ |
| ISSN: | 0888-8809 |
| DOI: | 10.1210/me.2011-1267⟩ |
| Popis: | FSH is a key hormonal regulator of Sertoli cell secretory activity, required to optimize sperm production. To fulfil its biological function, FSH binds a G protein-coupled receptor, the FSH-R. The FSH-R-transduced signaling network ultimately leads to the transcription or down-regulation of numerous genes. In addition, recent evidence has suggested that FSH might also regulate protein translation. However, this point has never been demonstrated conclusively yet. Here we have addressed this issue in primary rat Sertoli cells endogenously expressing physiological levels of FSH-R. We observed that, within 90 min of stimulation, FSH not only enhanced overall protein synthesis in a mammalian target of rapamycin-dependent manner but also increased the recruitment of mRNA to polysomes. m7GTP pull-down experiments revealed the functional recruitment of mammalian target of rapamycin and p70 S6 kinase to the 5′cap, further supported by the enhanced phosphorylation of one of p70 S6 kinase targets, the eukaryotic initiation factor 4B. Importantly, the scaffolding eukaryotic initiation factor 4G was also recruited, whereas eukaryotic initiation factor 4E-binding protein, the eukaryotic initiation factor 4E generic inhibitor, appeared to play a minor role in translational regulations induced by FSH, in contrast to what is generally observed in response to anabolic factors. This particular regulation of the translational machinery by FSH stimulation might support mRNA-selective translation, as shown here by quantitative RT-PCR amplification of the c-fos and vascular endothelial growth factor mRNA but not of all FSH target mRNA, in polysomal fractions. These findings add a new level of complexity to FSH biological roles in its natural target cells, which has been underappreciated so far. |
| Databáze: | OpenAIRE |
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