Differences in aromatase expression and steroid hormone concentrations in the reproductive tissues of male domestic turkeys (Meleagris gallopavo) with white and yellow semen
| Autor: | Barbara Bilińska, Malgorzata Kotula-Balak, Jan Jankowski, Malgorzata Brzoskwinia, Andrzej Ciereszko, Laura Pardyak, Alicja Kamińska, Anna Hejmej |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Turkeys steroid hormones medicine.drug_class medicine.medical_treatment Blotting Western Semen Real-Time Polymerase Chain Reaction Andrology 03 medical and health sciences Aromatase Testis medicine turkey Animals Testosterone Gonadal Steroid Hormones Receptor yellow semen syndrome Epididymis testes-epididymis-ductus deferens Estradiol biology Reproduction General Medicine Androgen Up-Regulation Steroid hormone 030104 developmental biology medicine.anatomical_structure Receptors Estrogen Animals Domestic biology.protein aromatase gene expression Animal Science and Zoology Food Science Hormone |
| Zdroj: | British Poultry Science. 59:591-603 |
| ISSN: | 1466-1799 0007-1668 |
| DOI: | 10.1080/00071668.2018.1483576 |
| Popis: | 1. To show hormonal differences between male turkeys with yellow semen syndrome (YSS) and white, normal semen (WNS), the expression of aromatase, oestrogen receptor α (ERα), and oestrogen receptor β (ERβ) as well as testosterone and oestradiol concentrations in YSS and WNS testes, epididymis, and ductus deferens were examined. 2. To measure gene expression levels of aromatase and oestrogen receptors (ERs), three complementary techniques (real-time PCR, Western blot, and immunohistochemistry) were used, whereas steroid hormone levels were determined radio-immunologically. 3. Upregulation of aromatase and ERα mRNAs in YSS testes (P |
| Databáze: | OpenAIRE |
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