Circaea mollis Siebold & Zucc. Alleviates postmenopausal osteoporosis in a mouse model via the BMP-2/4/Runx2 pathway

Autor: Chang Ho Lee, Gyhye Yoo, Chu Won Nho, Ji hye Park, Yang Ju Son
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
medicine.medical_specialty
Runt-related transcription factor 2
Bone Morphogenetic Protein 2
Core Binding Factor Alpha 1 Subunit
Biology
Bone morphogenetic protein 2
Bone resorption
Mice
03 medical and health sciences
0302 clinical medicine
Postmenopausal osteoporosis
Western blot
Osteoclast
Internal medicine
medicine
Animals
Humans
Osteoporosis
Postmenopausal
Bone morphogenetic protein 2/4
Bone mineral
Osteoblasts
medicine.diagnostic_test
Osteoblast differentiation
Plant Extracts
Circaea mollis Siebold & Zucc
Cell Differentiation
Osteoblast
lcsh:Other systems of medicine
lcsh:RZ201-999
Mice
Inbred C57BL
RUNX2
Disease Models
Animal
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Complementary and alternative medicine
Ovariectomized rat
Female
030217 neurology & neurosurgery
Research Article
Zdroj: BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-11 (2020)
BMC Complementary Medicine and Therapies
ISSN: 2662-7671
Popis: Background Circaea mollis Sieb. & Zucc. has been used as a traditional herbal medicine in Hani Ethnopharmacy and possesses anti-arthritic activities. This study aimed to investigate the effect of Circaea mollis Siebold & Zucc on postmenopausal osteoporosis. Methods For in vitro study, MCF7 breast cancer cells and MC3T3-E1 pre-osteoblast cells were utilized to estimate estrogenic and osteogenic activity. Osteoblastic markers were measured by western blot and real-time PCR. For in vivo study, female mature C57BL/6 mice were ovariectomized and oral administrated with 10 mg/kg and 40 mg/kg of EECM respectively. Results EtOH extract of Circaea mollis Siebold & Zucc. (EECM) increased alkaline phosphatase activity and osteoblast marker levels at day 7 during differentiation of mouse preosteoblasts. EECM reduced osteoclast differentiation and bone resorption in an osteoblast-osteoclast primary co-culture system. In ovariectomized mice, EECM prevented the decrease in bone mineral density and recovered OSX and Runx2 via BMP2/4, Smad1/5/9 and p38. Conclusions The results suggest that EECM may be effective in preventing bone loss, offering a promising alternative for the nutritional management of postmenopausal osteoporosis.
Databáze: OpenAIRE
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