Gene expressions and mechanical functions of α1-adrenoceptor subtypes in mouse ureter
| Autor: | Yasue Kubota, Yoshitaka Tomiyama, Yoshinobu Yamazaki, Hiroshi Kusama, Kenjiro Kohri, Yasunori Itoh, Yuji Hoyano, Shinya Kobayashi |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Indoles Epinephrine Urology Urinary stone Gene Expression Biology α1 adrenergic receptor Piperazines Mice Norepinephrine Ureter Receptors Adrenergic alpha-1 Gene expression medicine Animals RNA Messenger Gene Mice Inbred ICR Messenger RNA Prazosin Mouse Ureter α1 adrenoceptor Cell biology medicine.anatomical_structure Adrenergic alpha-1 Receptor Antagonists Muscle Contraction |
| Zdroj: | World Journal of Urology. 27:775-780 |
| ISSN: | 1433-8726 0724-4983 |
| DOI: | 10.1007/s00345-009-0396-y |
| Popis: | This study was performed to characterize the α1-adrenoceptor subtypes in mouse ureters as regards gene expressions and contractile functions.The mRNAs for these subtypes were quantified by the real-time quantitative reverse transcription polymerase chain reaction. In a functional study, α1-adrenoceptor antagonists were evaluated against the noradrenaline-induced contraction in mouse isolated ureteral preparations.In mouse ureter, the relative mRNA expression levels for α1a-, α1b- and α1d-adrenoceptors were 74.5, 14.3 and 11.2%, respectively. Adrenaline and noradrenaline each produced a concentration-dependent phasic contraction (pD 2 values, 5.73±0.05 and 5.69±0.06, respectively). Prazosin (non-selective α1-adrenoceptor antagonist), silodosin (selective α1A-adrenoceptor antagonist), and BMY-7378 (selective α1D-adrenoceptor antagonist) all shifted the concentration–response curve for noradrenaline to the right, the rank order of potencies (apparent pA 2 values) being silodosin (9.32±0.11)prazosin (8.55±0.10)BMY-7378 (6.06±0.15). The α1A-adrenoceptor antagonist silodosin was thus much more effective than the α1D-adrenoceptor antagonist BYM-7378.Our results demonstrate that in mouse ureters: the mRNA for the α1A-adrenoceptor was more prevalent than those for the α1B- and α1D-adrenoceptors, and that among these subtypes, the α1A-adrenoceptor plays the major role in noradrenaline-induced contraction. |
| Databáze: | OpenAIRE |
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