Effect of irrigation solutions for arthroscopic surgery on intraarticular tissue: Comparison in human meniscus-derived primary cell culture between lactate Ringer's solution and saline solution
| Autor: | Tatsuo Mae, Takahiro Ochi, Ken Nakata, Takahide Miyama, Hirotaka Shinjo, Masayuki Hamada, Shuji Horibe, Konsei Shino, Norimasa Nakamura, Hideki Yoshikawa |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male medicine.medical_specialty Ringer's Lactate Adolescent Transcription Genetic medicine.medical_treatment Gene Expression Sodium Chloride Biology Meniscus (anatomy) Cell morphology Menisci Tibial Collagen Type I Culture Media Serum-Free Cell membrane Arthroscopy medicine Irrigation Solutions Humans HSP70 Heat-Shock Proteins Lectins C-Type Orthopedics and Sports Medicine Aggrecans RNA Messenger Therapeutic Irrigation Collagen Type II Saline Cells Cultured Aggrecan Extracellular Matrix Proteins Middle Aged Surgery Procollagen peptidase medicine.anatomical_structure Cell culture Female Proteoglycans Isotonic Solutions |
| Zdroj: | Journal of Orthopaedic Research. 20:1305-1310 |
| ISSN: | 1554-527X 0736-0266 |
| Popis: | In order to determine whether there is a difference in effect on cell morphology and function between two common arthroscopic irrigation solutions, primary cultures of cells derived from the surgically excised human menisci were incubated for 3 or 6 h in lactated Ringer’s solution, isotonic sodium chloride solution, or serum-free cell culture medium (negative-control condition). Cell integrity was blindly evaluated by three independent examiners scoring photomicrographs of the cell cultures on a battery of five-point scales for abnormality of cell shape, irregularity of cell membrane, change of cell size and cell density. Cell cultures were also quantitatively assayed by semi-quantitative reverse-transcription–polymerase-chain-reaction for mRNA of α1 (I) procollagen, α1 (II) procollagen, aggrecan and heat-shock protein 70 to assess functional consequences of exposure to the solutions. There was a statistically significant difference in cell integrity scores between either lactated Ringer’s solution or serum-free cell-culture medium and isotonic sodium chloride solution with greater damage to cells displayed. Scores for lactated Ringer’s solution did not differ from those for serum-free cell-culture medium. There were no significant differences in mRNA expression level among the treatment conditions. It was concluded that the lactated Ringer’s solution better maintained human meniscus cell integrity than the isotonic saline. |
| Databáze: | OpenAIRE |
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