Description of a tandem repeated DNA sequence of Echinostoma caproni and methods for its detection in snail and plankton samples
| Autor: | Bernhard Haberl, J. Hertel, Joseph Hamburger, Wilfried Haas |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Snails
Zoology Biomphalaria Snail Sensitivity and Specificity Pulmonata law.invention Species Specificity law Echinostoma biology.animal Animals Humans Biomphalaria glabrata Polymerase chain reaction Disease Reservoirs Base Sequence biology Ecology DNA Helminth Plankton biology.organism_classification Infectious Diseases Tandem Repeat Sequences Larva Planorbidae Animal Science and Zoology Parasitology Schistosoma mansoni Trematoda |
| Zdroj: | Parasitology. 126:443-449 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182003003093 |
| Popis: | Echinostome larval stages in the snail have a great potential as effective competitors for the control of schistosomes and adult worms can cause painful intestinal diseases in humans. Ecology and transmission of the larval stages of trematodes are poorly understood, especially because their identification in field-collected samples by microscopy is difficult. We cloned, sequenced and analysed a 192 bp tandem repreated DNA sequence of Echinostoma caproni (EcSau3A), an often discussed antagonist of Schistosoma mansoni in Biomphalaria snails. PCR primers against this sequence can detect less than 10 fg of E. caproni DNA, 2 miracidia in snails 1 day p.i., 1 metacercaria in 50 mg snail tissue and 1 cercaria in 50 mg plankton with high specificity. Methods described in this study can support the discovery of fundamental ecological principles on distribution, host specificity and epidemiology of E. caproni larvae under field conditions. |
| Databáze: | OpenAIRE |
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