Polysaccharide microarrays for polysaccharide-platelet-derived-growth-factor interaction studies
| Autor: | Latifa Dahri-Correia, Jose Correia, Oleg Melnyk, Guy Dubreucq, Olivier Carion, Julien Lefebvre |
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| Rok vydání: | 2006 |
| Předmět: |
Platelet-derived growth factor
Microarray Surface Properties medicine.medical_treatment Becaplermin Polysaccharide Biochemistry chemistry.chemical_compound Peptide Library Polysaccharides medicine Humans Peptide library Molecular Biology Cell Proliferation chemistry.chemical_classification Platelet-Derived Growth Factor Growth factor Organic Chemistry Dextrans Proto-Oncogene Proteins c-sis Fibroblasts Microarray Analysis In vitro Molecular Weight Dextran chemistry Molecular Medicine Wound healing |
| Zdroj: | Chembiochem : a European journal of chemical biology. 7(5) |
| ISSN: | 1439-4227 |
| Popis: | Polysaccharide microarrays have great potential for the high-throughput analysis of polysaccharide-protein interactions. Here we demonstrate that a polysaccharide microarray prepared by printing a library of dextran polymers derivatized by methylcarboxylate, benzylamide, and sulfate groups (DMCBSu compounds) on to glass slides permitted the rapid identification of a set of compounds able to interact with the platelet-derived growth factor BB (PDGF-BB) isoform, a growth factor involved in wound healing. Microarray interaction results were compared to the capacity of DMCBSu compounds to potentiate the in vitro PDGF-BB-induced proliferation of human dermal fibroblasts. |
| Databáze: | OpenAIRE |
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