Molecular cloning and expression vector construction of bovine TRIM28
Autor: | Ma X, Su Ly, Dong Xq, Wang Cf, Zhang Ml, Song Bh, Ma Hx, Zhai Zc, Zhu Yr, Luan Wm, Yang Sb |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Genetic Vectors Molecular cloning Green fluorescent protein 03 medical and health sciences Genetics Fluorescence microscope Animals Cloning Molecular Molecular Biology Gene Cells Cultured Messenger RNA Expression vector Chemistry Ovary fungi General Medicine Transfection Fibroblasts Molecular biology Repressor Proteins 030104 developmental biology Lipofectamine Cattle Female |
Zdroj: | Genetics and Molecular Research. 15 |
ISSN: | 1676-5680 |
DOI: | 10.4238/gmr.15028793 |
Popis: | The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group. The TIMR28 gene has been successfully transferred into bovine fetal fibroblasts. |
Databáze: | OpenAIRE |
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