The ability of multipotent mesenchymal stromal cells from the bone marrow of patients with leukemia to maintain normal hematopoietic progenitor cells

Autor:	Nataliya Petinati, Oleg Shukhov, Anna G. Turkina, Ekaterina Chelysheva, Nina Drize, Irina N. Shipounova, Elena N. Parovichnikova, Larisa A. Kuzmina, Tamara Sorokina, Alexey Bigildeev, Valery G. Savchenko
Rok vydání:	2016
Předmět:	Adult Male 0301 basic medicine Myeloid Stromal cell Adolescent medicine.medical_treatment Cell Culture Techniques Multipotent Mesenchymal Stromal Cells Cell Count Young Adult 03 medical and health sciences Bone Marrow hemic and lymphatic diseases Humans Medicine Cells Cultured Cobblestone Area-Forming Cells Chemotherapy Leukemia business.industry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Hematology General Medicine Middle Aged Hematopoietic Stem Cells medicine.disease Hematopoiesis 030104 developmental biology medicine.anatomical_structure Case-Control Studies Immunology Female Bone marrow business
Zdroj:	European Journal of Haematology. 97:245-252
ISSN:	0902-4441
DOI:	10.1111/ejh.12713
Popis:	BACKGROUND The development of leukemia impairs normal hematopoiesis and marrow stromal microenvironment. The aim of the investigation was to study the ability of multipotent mesenchymal stromal cells (MSCs) derived from the bone marrow of patients with leukemia to maintain normal hematopoietic progenitor cells. METHODS MSCs were obtained from the bone marrow of 14 patients with acute lymphoblastic (ALL), 25 with myeloid (AML), and 15 with chronic myeloid (CML) leukemia. As a control, MSCs from 22 healthy donors were used. The incidence of cobblestone area forming cells (CAFC 7-8 d) in the bone marrow of healthy donor cultivated on the supportive layer of patients MSCs was measured. RESULTS The ability of MSCs from AML and ALL patients at the moment of diagnosis to maintain normal CAFC was significantly decreased when compared to donors. After chemotherapy, the restoration of ALL patients' MSCs functions was slower than that of AML. CML MSCs maintained CAFC better than donors' at the moment of diagnosis and this ability increased with treatment. CONCLUSIONS The ability of patients' MSCs to maintain normal hematopoietic progenitor cells was shown to change in comparison with MSCs from healthy donors and depended on nosology. During treatment, the functional capacity of patients' MSCs had been partially restored.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5de77238ea6519228418ffeb836095c7 https://doi.org/10.1111/ejh.12713 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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