Shearm-stress causes polarized change in cytoplasmic calcium concentration in human umbilical vein endothelial cells (HUVECs)
| Autor: | Tomio Kawasaki, Masataka Ikeda, Hideo Ariyoshi, Norihide Yoshikawa, Masato Sakon, Morito Monden |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cytoplasm
Umbilical Veins Cytoplasmic calcium Physiology Cell In Vitro Techniques Biology Umbilical vein Intracellular ca Adenosine Triphosphate Confocal laser scanning microscopy medicine Humans Channel blocker Molecular Biology Fluorescent Dyes Aniline Compounds Microscopy Confocal Cell Biology Cell biology Membrane medicine.anatomical_structure Xanthenes Biophysics Calcium Calcium Channels Endothelium Vascular Stress Mechanical Intracellular Signal Transduction |
| Zdroj: | Cell Calcium. 22:189-194 |
| ISSN: | 0143-4160 |
| DOI: | 10.1016/s0143-4160(97)90012-9 |
| Popis: | Using a newly developed, parallel-plate flow-chamber for confocal laser scanning microscopy (CLSM), we studied the distribution and temporal changes in intracellular Ca 2+ concentration ([Ca 2+ ] i ) in individual HUVECs stimulated by shear-stress. In the presence of ATP, shear-stress (1–10 dyne/cm 2 ) caused a rise in [Ca 2+ ] i , whereas no such response was observed in the absence of ATP or in the presence of Ni 2+ , a nonspecific, plasma membrane Ca 2+ channel blocker. These results suggest that both ATP and Ca 2+ influx are essential for the increase in [Ca 2+ ] i in response to shear stress at less than 10 dyne/cm 2 . Analysis of [Ca 2+ ] i distribution revealed a repetitive intracellular ‘Ca 2+ wave’ originating from the upstream edge of the cell in some populations of HUVECS, which was transmitted to the downstream of the cell. The polarized [Ca 2+ ] i response induced by shear-stress might be integral to polarized cellular reactions such as remodeling of endothelial lining. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect