Conventional mechanical ventilation of healthy lungs induced pro-inflammatory cytokine gene transcription
| Autor: | Antoine Roch, Yves Jammes, Osamu Kajikawa, Thomas R. Martin, Fabienne Bregeon, S. Delpierre, Jérôme Pugin, Henry Portugal, Amapola Autillo-Touati, Eric Ghigo, Jean-Pierre Auffray |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Pathology Transcription Genetic Physiology medicine.medical_treatment Leukocyte Count Internal medicine medicine Animals RNA Messenger Respiratory system Lung Tidal volume Positive end-expiratory pressure Mechanical ventilation Analysis of Variance medicine.diagnostic_test business.industry Pulmonary Gas Exchange Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Oxygenation Pneumonia respiratory system Respiration Artificial respiratory tract diseases Bronchoalveolar lavage Endocrinology medicine.anatomical_structure Arterial blood Cytokines Rabbits Inflammation Mediators business Bronchoalveolar Lavage Fluid |
| Zdroj: | Respiratory physiologyneurobiology. 132(2) |
| ISSN: | 1569-9048 |
| Popis: | We investigated the potential inflammatory reaction induced by mechanical ventilation (MV) using 10 ml/kg tidal volume and no positive end-expiratory pressure (PEEP) in control (C, n = 8), spontaneously breathing (SB, n = 12) and mechanically ventilated (MV, n = 12) rabbits with normal lungs. After 6 h (MV and SB groups) or immediately (C group), lungs were removed for measurement of wet-to-dry (W/D) weight ratio and for bronchoalveolar lavage (BAL). Pulmonary mechanics were also studied. MV animals developed a modest but significant (P < 0.01) impairment of arterial blood oxygenation and had higher W/D lung weight ratio than C ones. In MV group, BAL macrophage count was greater (P < 0.05) than in SB one. MV induced an upregulation of MCP-1, TNF-alpha, and IL-1beta gene transcription (mRNAs), without significant elevation of the corresponding protein cytokines in the BAL supernatant, except for MCP-1 (P < 0.05). These data suggest that MV, even using moderate tidal volume, elicits a pro-inflammatory stimulus to the lungs. |
| Databáze: | OpenAIRE |
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