Spontaneous reversion of Mycobacterium abscessus from a smooth to a rough morphotype is associated with reduced expression of glycopeptidolipid and reacquisition of an invasive phenotype
| Autor: | Susan T. Howard, Anny Alsup, Roberto Kolter, Judith Recht, Xiuhua Pang, C. Rick Lyons, Thomas F. Byrd, Elizabeth Rhoades |
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| Rok vydání: | 2006 |
| Předmět: |
Reversion
Mycobacterium Infections Nontuberculous Motility Virulence Mice SCID Biology Mycobacterium abscessus Microbiology Monocytes Cell wall Mice Serial passage Animals Humans Lung Cells Cultured Glycopeptides Biofilm Nontuberculous Mycobacteria biology.organism_classification Phenotype Culture Media Disease Models Animal Biofilms Glycolipids |
| Zdroj: | Microbiology. 152:1581-1590 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.28625-0 |
| Popis: | Mycobacterium abscessusis an increasingly important cause of human disease; however, virulence determinants are largely uncharacterized. Previously, it was demonstrated that a rough, wild-type human clinical isolate (390R) causes persistent, invasive infection, while a smooth isogenic mutant (390S) has lost this capability. During serial passage of 390S, a spontaneous rough revertant was obtained, which was named 390V. This revertant regained the ability to cause persistent, invasive infection in human monocytes and the lungs of mice. Glycopeptidolipid (GPL), which plays a role in environmental colonization, was present in abundance in the cell wall of 390S, and was associated with sliding motility and biofilm formation. In contrast, a marked reduction in the amount of GPL in the cell wall of 390R and 390V was correlated with cord formation, a property associated with mycobacterial virulence. These results indicate that the ability to switch between smooth and rough morphologies may allowM. abscessusto transition between a colonizing phenotype and a more virulent, invasive form. |
| Databáze: | OpenAIRE |
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