Comparative in vitro activity of plazomicin and older aminoglyosides against Enterobacterales isolates; prevalence of aminoglycoside modifying enzymes and 16S rRNA methyltransferases

Autor: Aydemir, Sohret, Sari, Ayse Nur, Gulay, Zeynep, AKPOLAT, NEZAHAT, ÇELİK, CEM, Bakici, Zahir, EVREN, EBRU, KARAHAN, ZEYNEP CEREN, Kararuna, Onur, ÜLGER, NURVER, Soyletir, Guner, ALTUN, BELGİN, SANCAK, BANU, AKSU, MEHMET BURAK, Celik, Tugce, Cavusoglu, Iffet, ÇAKAR, ASLI, HASDEMİR GÖKBOĞA, MÜNEVVER UFUK, Gur, Deniz, Tuncer, Inci, ÇAYCI, YELİZ TANRIVERDİ, BİRİNCİ, ASUMAN, ÖZKAYA, ESRA, AYDIN, FARUK, Koksal, Fauna, Buyuktas, Ayse, ZER, YASEMİN, PARKAN, ÖMÜR MUSTAFA, KILIÇ, HÜSEYİN, ÇİLLİ, FATMA FERİHA
Přispěvatelé: Ege Üniversitesi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Klebsiella
Serratia
Turkey
Aminoglycoside modifying enzyme
chemistry.chemical_compound
rmtF
0302 clinical medicine
16S rRNA methyltransferase
rmtE
rmtC
RNA
Ribosomal
16S
rmtB
rmtA
Prevalence
030212 general & internal medicine
Aminoglycoside resistance
OXA-48
Broth microdilution
Enterobacteriaceae Infections
General Medicine
Enterobacter
Infectious Diseases
Amikacin
Sisomicin
medicine.drug
Microbiology (medical)
030106 microbiology
Plazomicin
Microbial Sensitivity Tests
Biology
NDM-1
beta-Lactamases
Microbiology
03 medical and health sciences
armA
Bacterial Proteins
Enterobacteriaceae
Acetyltransferases
Drug Resistance
Bacterial
medicine
Humans
E. coli
Methyltransferases
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Aminoglycosides
chemistry
Netilmicin
Enterobacter cloacae
Popis: Comparative in vitro activity of plazomicin and 4 older aminoglycosides was evaluated with broth microdilution in 714 blood isolates from 14 hospitals in Turkey. Isolates included Escherichia coli (n=320), Klebsiella spp. (n=294), Enterobacter spp. (n =69), Serratia marcescens (n=20). and Citrobacter spp. (n=11). Isolates resistant to older aminoglycosides (n=240) were screened for aminoglycoside modifying enzyme genes: aac(6')-Ib, aac(3)-Ia, aac(3)-IIa, ant(2 '')-Ia. Isolates with high MICs for plazomicin (n=41) were screened for 165 rRNA methyltransferase genes (armA, rmtA, rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH, npmA) and 2 carbapenemase genes (blaOXA-48, blaNDM-1). Overall, resistance to plazomicin, amikacin, netilmicin, gentamicin, and tobramycin was 7.7%, 7.4%, 31.5%, 32.9%, and 34.7%, respectively. aac(6')-Ib and aac(3)-IIa were the most common AME genes. Co-occurrence of blaNDM-1 with armA and rmtC and blaOXA-48 with armA was striking. Enterobacter cloacae carrying rmtC+blaNDM-1, S. marcescens with armA+blaOXA-48, and rmtF+ blaOXA-48 in K. pneumoniae were reported for the first time. (C) 2020 Elsevier Inc. All rights reserved.
Achaogen Inc., CA, USA
The authors would like to acknowledge Achaogen Inc., CA, USA, for providing plazomicin and partly funding the tests for in vitro susceptibilities and characterization of aminoglycoside resistance genes.
Databáze: OpenAIRE
Externí odkaz: