Effects of aflatoxin B1 on oxidative stress markers and apoptosis of spleens in broilers
| Autor: | Yi Geng, Xi Peng, Fengyuan Wang, Kejie Chen, Hengmin Cui, Jingxin Yuan, Shibin Yuan, Jing Fang, Jin Chen, Zhengli Chen |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
0301 basic medicine
Male Aflatoxin B1 Health Toxicology and Mutagenesis Glutathione reductase Apoptosis Toxicology medicine.disease_cause Andrology Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Random Allocation Malondialdehyde medicine Splenocyte In Situ Nick-End Labeling Animals Lymphocytes Annexin A5 Immunosuppression Therapy biology Public Health Environmental and Occupational Health Glutathione Catalase Molecular biology Oxidative Stress 030104 developmental biology Glutathione Reductase chemistry biology.protein Lipid Peroxidation Chickens Oxidative stress Biomarkers Spleen Peroxidase |
| Zdroj: | Toxicology and industrial health. 32(2) |
| ISSN: | 1477-0393 |
| Popis: | The purpose of the present study was to investigate the oxidative damage and apoptosis induced by aflatoxin B1 (AFB1) in spleen of broilers. A total of 200 one-day-old avian male broilers were randomly divided into 4 equal groups of 50 each and were fed for 21 days as follows: a control diet and three AFB1 diets containing 0.15, 0.3, and 0.6 mg AFB1/kg diet. Consumption of AFB1 diets induced oxidative stress in the spleen of chicken as evidenced by reduced glutathione peroxidase, glutathione reductase, and catalase activities, decreased glutathione contents, and increased malondialdehyde contents in explaining the pathogenesis. Flow cytometer method and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling assay revealed that the apoptotic splenocytes were increased in AFB1 groups. The results suggest that AFB1 induced excessive apoptosis of splenic lymphocytes, which is correlated with increased oxidative stress. The present results may be helpful for explaining the pathogenesis of AFB1-induced immunosuppression. |
| Databáze: | OpenAIRE |
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