Determination of maternal and foetal distribution of cis- and trans-permethrin isomers and their metabolites in pregnant rats by liquid chromatography tandem mass spectrometry (LC-MS/MS)
| Autor: | Paulo Marcelo, Sylvie Baltora-Rosset, Véronique Bach, Franck Robidel, Anthony Lecomte, Céline Brochot, Aurélie Corona, Florence Anna Zeman, Serge Pilard, Stéphane Personne |
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| Přispěvatelé: | Institut National de l'Environnement Industriel et des Risques (INERIS), Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de l'Environnement Industriel et des Risques, Université de Picardie Jules Verne (UPJV), Ecologie et Dynamique des Systèmes Anthropisés - UMR CNRS 7058 (EDYSAN), Centre National de la Recherche Scientifique (CNRS)-Université de Picardie Jules Verne (UPJV) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Insecticides Carboxylic acid PYRETHROIDS 02 engineering and technology Urine PESTICIDES/ENDOCRINE DISRUPTORS Tandem mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry Matrix (chemical analysis) Rats Sprague-Dawley Fetus Isomerism Liquid chromatography–mass spectrometry Tandem Mass Spectrometry parasitic diseases PERMETHRIN medicine Toxicokinetics Animals Sample preparation LC-MS/MS chemistry.chemical_classification Chromatography Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Rats PREGNANCY [SDV.TOX]Life Sciences [q-bio]/Toxicology RAT Female 0210 nano-technology Permethrin medicine.drug Chromatography Liquid |
| Zdroj: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry, Springer Verlag, 2019, 411 (30), pp.8043-8052. ⟨10.1007/s00216-019-02157-7⟩ |
| ISSN: | 1618-2642 1618-2650 |
| Popis: | We developed a method to quantify cis-permethrin and trans-permethrin and their metabolites in several biological matrices in pregnant rats and foetuses using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The objective was to quantify cis-permethrin and trans-permethrin in faeces, kidney, mammary gland, fat and placenta in mothers and in both maternal and foetal blood, brain and liver. The metabolites cis-3-(2,2-dichlorovinyl)-2,2-dimethyl-(1-cyclopropane) carboxylic acid (cis-DCCA), trans-3-(2,2-dichlorovinyl)-2,2-dimethyl-(1-cyclopropane) carboxylic acid (trans-DCCA) and 3-phenoxybenzoic acid (3-PBA) were measured in blood, liver and urine. Sample preparation was performed by liquid-liquid extraction. A purification step was not carried out except for the more complex biological samples (fat, mammary glands and faeces). Validation parameters including specificity, linearity, matrix effect, limits of quantification (LOQs), accuracy and precision were evaluated. The recoveries of target compounds ranged from 47 to 136%. LOQs were in the range 4 to 80 ng/mL for permethrin isomers and 4 to 800 ng/mL for their respective metabolites. Intra- and inter-batch precision and accuracy in matrix were better than 15%. The validated method was applied in a preliminary toxicokinetic study in pregnant rats with oral dosing of 50 mg/kg permethrin. In pregnant rats, permethrin isomers and their metabolites were quantified in all requested matrices except maternal liver and blood for trans-permethrin and cis-DCCA respectively. In foetuses, cis- and trans-permethrin were also quantified, demonstrating that the method is suitable for the analysis of foetal distribution of permethrin in toxicokinetic studies. |
| Databáze: | OpenAIRE |
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