Sunfish amphiphiles: Conceptually new carriers for DNA delivery
| Autor: | Hulst, Ron, Muizebelt, I., Oosting, P., van der Pol, C., Wagenaar, Anno, Smisterova, J., Bulten, E., Driessen, C., Hoekstra, D, Engberts, J.B.F.N., Šmisterová, J. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
VECTOR
MEMBRANE-FUSION in vitro transfection TRANSFECTION chemistry.chemical_compound Amphiphile Physical and Theoretical Chemistry amphiphiles BIODEGRADABLE PYRIDINIUM AMPHIPHILES vesicles SOLVENT GENE-THERAPY DEATH Chemistry Vesicle Organic Chemistry Cationic polymerization Lipid bilayer fusion Biological membrane Transfection DNA SERIES Biochemistry COMPLEXES Pyridinium controlled release BEHAVIOR LIPIDS |
| Zdroj: | European Journal of Organic Chemistry, 835-849. WILEY-V C H VERLAG GMBH ISSUE=4;STARTPAGE=835;ENDPAGE=849;TITLE=European Journal of Organic Chemistry |
| Popis: | A conceptually new class of cationic amphiphiles, Sunfish amphiphiles, designed for the delivery of genes into cells is introduced. Sunfish amphiphiles have two hydrophobic tails, connected at the 4- and the N-position to the cationic pyridinium headgroup. Two extreme morphologies visualised by backfolding and combining of both tails at one site (matching situation) or unfolding of the tails at distinct interaction sites at biological membranes will lead to considerable differences in morphological behaviour. The underlying rationale allows controlled release by using this morphological alteration of the Sunfish/helper-lipid/DNA complex (lipoplex). The often excellent transfection efficiencies are probably related to these morphological changes. In addition, the Sunfish amphiphiles possess low toxicities, resulting in high cell survival after internalisation. The underlying rationale, design, synthesis and in vitro transfection potential are discussed in detail. Moreover, some physico-chemical characteristics of the Sunfish amphiphiles have been studied. (C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|