Aberrant KIF20A Expression Is Associated with Adverse Clinical Outcome and Promotes Tumor Progression in Prostate Cancer
| Autor: | Junpeng Ji, Xiaoqing Li, Yuanjie Niu, Zhiqun Shang, Ciman Chai, Tianyu Shen, Zheng Zhang, Changying Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biochemical recurrence Male Article Subject Clinical Biochemistry Kinesins Mice Nude Adenocarcinoma medicine.disease_cause 03 medical and health sciences Prostate cancer Mice 0302 clinical medicine Prostate Cell Movement Cell Line Tumor Genetics medicine Biomarkers Tumor Animals Humans MTT assay Molecular Biology Aged Cell Proliferation lcsh:R5-920 business.industry Biochemistry (medical) Prostatic Neoplasms General Medicine medicine.disease 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Tumor progression 030220 oncology & carcinogenesis Cancer research Immunohistochemistry Carcinogenesis business lcsh:Medicine (General) Research Article |
| Zdroj: | Disease Markers Disease Markers, Vol 2019 (2019) |
| ISSN: | 1875-8630 0278-0240 |
| Popis: | Purpose. KIF20A is essential in the process of spindle assembly and cytokinesis regulation. The role of KIF20A during tumorigenesis and tumor development has been well studied in several cancers. But the association between the KIF20A clinical role and prostate cancer (PCa) has not been reported yet. In this study, we investigated its potential prognostic effect and its role in progression of prostate cancer. Methods. Real-time quantitative polymerase chain reaction and Western blots were used to investigate the KIF20A transcription and translation levels in 7 pairs of fresh PCa tissue and adjacent normal prostate tissue. Immunohistochemistry (IHC) was used to investigate the KIF20A protein level in 114 PCa tissue samples. Bioinformatics analysis was performed to analyze the effect of KIF20A in oncologic prognosis in PCa patients. MTT assay, transwell assay, and colony formation assay in vitro and tumor formation assay in vivo were performed to evaluate the biological behavior of KIF20A in prostate cancer. Results. KIF20A was significantly elevated in tumor tissue compared with normal prostate tissue at both the mRNA and the protein level. High expression of KIF20A at the protein level was correlated with adverse clinicopathological features. Bioinformatics analysis showed that the high KIF20A expression group has a poor biochemical recurrence- (BCR-) free survival. Knocking down KIF20A suppressed the proliferation, migration, and invasion of the prostate cancer cell both in vitro and in vivo. Conclusions. Our data demonstrated that the high expression of KIF20A was associated with poor clinical outcome and targeting KIF20A could reduce proliferation, migration, and invasion of the prostate cancer cell, indicating that KIF20A might be a potential prognostic and therapeutic target for PCa patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text