Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells
| Autor: | F A van der Hoorn, H Fan, R A Bosselman, Inder M. Verma, M Jones, A Berns |
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| Rok vydání: | 1980 |
| Předmět: |
Genes
Viral EcoRI Molecular cloning law.invention chemistry.chemical_compound Nucleic acid thermodynamics Mice law Animals Cloning Molecular Nuclease Multidisciplinary biology Nucleic Acid Hybridization DNA DNA Restriction Enzymes Virology Molecular biology Bacteriophage lambda Mouse sarcoma virus Molecular Weight Restriction enzyme Microscopy Electron chemistry DNA Viral biology.protein Recombinant DNA Moloney murine leukemia virus Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 77(5) |
| ISSN: | 0027-8424 |
| Popis: | When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRI, a DNA fragment of 14.0 kilobases can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kilobase EcoRI DNA fragment was cloned in bacteriophage lambda. The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy. The results indicate that lambda . MTX-1 contains an uninterrupted stretch of 1.0 kilobase similar to that found in the M-MSV genome. |
| Databáze: | OpenAIRE |
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