Three-dimensional Imaging Coupled with Topological Quantification Uncovers Retinal Vascular Plexuses Undergoing Obliteration
| Autor: | Tzung K. Hsiai, Pierre J. Guihard, Kristina I. Boström, Lynn K. Gordon, Song Li, Alison Chu, Parinaz Abiri, Varun Gudapati, Jie Zheng, Kyung In Baek, Michel M. Sun, Xili Ding, Yichen Ding, Chih-Chiang Chang, Scott Meyer |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Primary and secondary plexus Oncology and Carcinogenesis Medicine (miscellaneous) Hyperoxia Retinal Neovascularization Topology 01 natural sciences Retina Oxygen induced retinopathy 010309 optics 03 medical and health sciences chemistry.chemical_compound Mice Imaging Three-Dimensional Pregnancy 0103 physical sciences medicine Animals Oxygen-induced retinopathy Pharmacology Toxicology and Pharmaceutics (miscellaneous) Plexus Vascular connectivity Retinal Vessels Retinal medicine.disease Vertical sprouts Mice Inbred C57BL Oxygen Disease Models Animal 030104 developmental biology Microvascular Network Three dimensional imaging chemistry Animals Newborn Light sheet fluorescence microscopy Retinal vasculature cardiovascular system Female Light-sheet fluorescence microscopy Retinopathy Research Paper |
| Zdroj: | Theranostics, vol 11, iss 3 Theranostics |
| Popis: | Introduction: Murine models provide microvascular insights into the 3-D network disarray seen in retinopathy and cardiovascular diseases. Light-sheet fluorescence microscopy (LSFM) has emerged to capture retinal vasculature in 3-D, allowing for assessment of the progression of retinopathy and the potential to screen new therapeutic targets in mice. We hereby coupled LSFM, also known as selective plane illumination microscopy, with topological quantification, to characterize the retinal vascular plexuses undergoing preferential obliteration. Method and Result: In postnatal mice, we revealed the 3-D retinal microvascular network in which the vertical sprouts bridge the primary (inner) and secondary (outer) plexuses, whereas, in an oxygen-induced retinopathy (OIR) mouse model, we demonstrated preferential obliteration of the secondary plexus and bridging vessels with a relatively unscathed primary plexus. Using clustering coefficients and Euler numbers, we computed the local versus global vascular connectivity. While local connectivity was preserved (p > 0.05, n = 5 vs. normoxia), the global vascular connectivity in hyperoxia-exposed retinas was significantly reduced (p < 0.05, n = 5 vs. normoxia). Applying principal component analysis (PCA) for auto-segmentation of the vertical sprouts, we corroborated the obliteration of the vertical sprouts bridging the secondary plexuses, as evidenced by impaired vascular branching and connectivity, and reduction in vessel volumes and lengths (p < 0.05, n = 5 vs. normoxia). Conclusion: Coupling 3-D LSFM with topological quantification uncovered the retinal vasculature undergoing hyperoxia-induced obliteration from the secondary (outer) plexus to the vertical sprouts. The use of clustering coefficients, Euler's number, and PCA provided new network insights into OIR-associated vascular obliteration, with translational significance for investigating therapeutic interventions to prevent visual impairment. |
| Databáze: | OpenAIRE |
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