Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis
| Autor: | W.T. Mason, A.J. Morton, B. Somasundaram, P.C. Emson, P.M. Lledo |
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| Rok vydání: | 1992 |
| Předmět: |
Calbindins
Genetic Vectors Gene Expression chemistry.chemical_element Calcium Pituitary neoplasm Transfection Cell membrane S100 Calcium Binding Protein G Tumor Cells Cultured medicine Animals Homeostasis Pituitary Neoplasms Patch clamp Ion transporter Chelating Agents Voltage-dependent calcium channel General Neuroscience Cell Membrane Electric Conductivity Rats Cell biology Cytosol Retroviridae medicine.anatomical_structure chemistry Biochemistry Calbindin 1 Calcium Channels Intracellular |
| Zdroj: | Neuron. 9:943-954 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/0896-6273(92)90246-a |
| Popis: | Previous work demonstrating the presence and differential distribution of Ca(2+)-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect the pituitary tumor cell line GH3, to generate CB-expressing GH3 cells and to investigate whether ionic channel activities as well as the concentration of intracellular free Ca2+ ([Ca2+]i) homeostasis could be altered by the presence of this Ca(2+)-binding protein. We show that CB-transfected GH3 cells exhibited lower Ca2+ entry through voltage-dependent Ca2+ channels and were better able to reduce [Ca2+]i transients evoked by voltage depolarizations than the wild-type parent cell line. These observations provide a mechanism by which CB may protect tissues against Ca(2+)-mediated excitotoxicity. |
| Databáze: | OpenAIRE |
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