Purification and characterization of sn-1-stearoyl-2-arachidonoylglycerol kinase from pig testes
| Autor: | S D Gardner, Sally Rose, Michael J.O. Wakelam, Andrew Paterson, Matthew N. Hodgkin, Ashley Martin |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Silver Staining Swine Biology Biochemistry Substrate Specificity Diglycerides Silver stain Sn-1-stearoyl-2-arachidonoylglycerol kinase Testis Animals Phosphorylation Molecular Biology chemistry.chemical_classification Blue dye Kinase Diradylglycerol Cell Biology Molecular biology Phosphotransferases (Alcohol Group Acceptor) Enzyme chemistry lipids (amino acids peptides and proteins) Chromatography Thin Layer Sphingomyelin Research Article Subcellular Fractions |
| Zdroj: | Biochemical Journal. 322:529-534 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3220529 |
| Popis: | 1-Stearoyl-2-arachidonoylglycerol (SAG) kinase was identified in the particulate fraction of pig testes. This activity was enriched by hydroxyapatite and blue dye chromatography. The enzyme was selective for polyunsaturated diradylglycerol species and activity was not modulated by other diradylglycerol species or sphingomyelin metabolites. Further purification resulted in the isolation of 55 and 50 kDa proteins that corresponded with SAG kinase activity. These results support the view that the phosphorylation of polyunsaturated diradylglycerol is regulated by structural determinants in the molecule. |
| Databáze: | OpenAIRE |
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