Occurrence of Echinococcus granulosus sensu lato and Other Taeniids in Bhutan
| Autor: | Tenzin Tenzin, Kinzang Dukpa, Phurpa Namgyel, Sonam Pelden, Yoenten Phuentshok, Puspa Maya Sharma, Nirmal Kumar Thapa, Chimi Jamtsho, Tshering Norbu, Krishna P Sharma, Lhatru Lhatru, Ratna B. Gurung, Pema Tshomo, Peter Deplazes, Cristian A. Alvarez Rojas, Tshewang Dema |
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| Přispěvatelé: | University of Zurich, Sharma, Puspa M, Deplazes, Peter |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
10078 Institute of Parasitology Veterinary medicine lcsh:Medicine 610 Medicine & health Taenia multiceps Biology 2726 Microbiology (medical) Article 600 Technology 2400 General Immunology and Microbiology parasitic diseases 1312 Molecular Biology Immunology and Allergy E. ortleppi Molecular Biology Gene Feces Genetic diversity General Immunology and Microbiology Haplotype lcsh:R 2725 Infectious Diseases Ribosomal RNA biology.organism_classification DNA extraction Coenurosis cystic echinococcosis Infectious Diseases Echinococcus 2723 Immunology and Allergy 570 Life sciences biology coenurosis |
| Zdroj: | Pathogens Pathogens, Vol 10, Iss 330, p 330 (2021) Volume 10 Issue 3 |
| ISSN: | 2076-0817 |
| Popis: | The present research shows the results of a national study documenting the occurrence and genetic diversity of Echinococcus and Taenia species across Bhutan. Environmental dog faecal samples (n = 953) were collected from 2016 to 2018 in all 20 Bhutanese districts, mainly in urbanised areas. Cystic echinococcosis cysts were isolated from 13 humans and one mithun (Bos frontalis). Isolation of taeniid eggs from faeces was performed by sieving/flotation technique, followed by DNA isolation, PCR and sequence analyses for species identification (gene target: small subunit of ribosomal RNA, rrnS). Haplotype diversity of E. granulosuss.s. was based on the sequence (1,609 bp) of the cox1 gene. A total of 67 out of 953 (7%) dog faecal samples were positive for at least one taeniid species. From the 670 free-roaming dog faecal samples, 40 (5.9%) were positive for taeniid DNA, 22 (3.2%) of them were identified as E. granulosuss.s. and four (0.5%) as E. ortleppi (G5). From the 283 faecal samples originating from yak-grazing areas, 27 (9.5%) were taeniid positive, including eight (2.8%) infected with E. granulosuss.s. and four (1.4%) with E. ortleppi. E. granulosuss.s. was identified in all isolates from human and the cyst from mithun. A haplotype network (cox1 gene) from E. granulosuss.s, including isolates from 12 dogs, two human and one mithun, revealed eight different haplotypes. The most common cox1 haplotype was the globally distributed Eg01, followed by Eg40 and Eg37 (previously described in China). Five new cox1 haplotypes (EgBhu1–5) originated from human, dogs, and a mithun were identified. The study indicated the contamination of urban areas and pastures with Echinococcus eggs in seven districts in Bhutan. The molecular characterisation of E. granulosuss.l. revealed different E. granulosuss.s. haplotypes as well as E. ortleppi. The transmission of T. multiceps was documented only in the western part of the country. Considering the zoonotic feature of E. granulosus s.s. and E. ortleppi and the economic impact of coenurosis caused by T. multiceps (also known as gid) in Bhutan, the findings of this study represent a significant contribution towards an epidemiological baseline for the establishment of a national control programme. |
| Databáze: | OpenAIRE |
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