Intracellular calcium mobilization is required for Sonic hedgehog signaling
| Autor: | Michael J. Jurynec, Dana Klatt Shaw, Erin Ritchie, David Grunwald, Derrick Gunther, Alexis A. Chagovetz |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell type Neural Tube Embryo Nonmammalian chemistry.chemical_element Calcium Biology General Biochemistry Genetics and Molecular Biology Calcium in biology Article 03 medical and health sciences 0302 clinical medicine Morphogenesis Animals Hedgehog Proteins Molecular Biology Zebrafish Body Patterning Ryanodine receptor Muscles Gene Expression Regulation Developmental Cell Biology Zebrafish Proteins biology.organism_classification Hedgehog signaling pathway Cell biology 030104 developmental biology chemistry Somites Signal transduction 030217 neurology & neurosurgery Intracellular Developmental Biology Signal Transduction |
| Popis: | Graded Shh signaling across fields of precursor cells coordinates patterns of gene expression, differentiation, and morphogenetic behavior as precursors form complex structures, such as the nervous system, the limb, and craniofacial skeleton. Here we discover that intracellular calcium mobilization, a process tightly controlled and readily modulated, regulates the level of Shh-dependent gene expression in responding cells and affects the development of all Shh-dependent cell types in the zebrafish embryo. Reduced expression or modified activity of Ryanodine Receptor (RyR) intracellular calcium release channels shifted the allocation of Shh-dependent cell fates in the somitic muscle and neural tube. Mosaic analysis revealed that RyR-mediated calcium mobilization is required specifically in Shh ligand-receiving cells. This work reveals that Ryanodine Receptor channels participate in intercellular signal transduction events. As modulation of RyR activity modifies tissue patterning, we hypothesize that alterations in intracellular calcium mobilization contribute to both birth defects and evolutionary modifications of morphology. |
| Databáze: | OpenAIRE |
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