Oxytocin induces mammary epithelium disruption and could stimulate epithelial cell exfoliation

Autor: Vanessa Lollivier, Lucile Hervé, H. Quesnel, Marion Boutinaud
Přispěvatelé: Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Université Bretagne Loire (UBL), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Cancer Research
épithélium
Contraction (grammar)
Lactose
Epithelium
chemistry.chemical_compound
Vasotocin
fluids and secretions
milking
ocytocine
dairy cows
Exfoliation
Udder
traite
Cross-Over Studies
cellule myoépithéliale
[SDV.BA]Life Sciences [q-bio]/Animal biology
food and beverages
04 agricultural and veterinary sciences
medicine.anatomical_structure
Milk
Oncology
Mammary Epithelium
vache laitière
Female
medicine.drug
skin
Mammary epithelial cell
Biology
Milking
Andrology
03 medical and health sciences
Mammary Glands
Animal
oxytocin
medicine
Dairy cow
Animals
Lactation
cellule épithéliale mammaire
Milk Ejection
0402 animal and dairy science
Myoepithelial cell
contraction
Epithelial Cells
Myoepithelial cell contraction
040201 dairy & animal science
030104 developmental biology
shrinkage
Oxytocin
chemistry
Cattle
Zdroj: Journal of Mammary Gland Biology and Neoplasia
Journal of Mammary Gland Biology and Neoplasia, Springer Verlag (Germany), 2018, 23 (3), pp.139-147. ⟨10.1007/s10911-018-9400-8⟩
ISSN: 1083-3021
1573-7039
Popis: Mammary epithelial cells (MEC) are exfoliated from the epithelium into milk, influencing the number of MEC present in the udder. This process is associated with epithelium integrity. The release of oxytocin (OT) induced by milking causes myoepithelial cell contraction, which, in turn, may stimulate MEC exfoliation through mechanical forces. To investigate the role of OT in MEC exfoliation, we inhibited or induced myoepithelial cell contraction by injecting the OT receptor antagonist atosiban (Ato) or a supraphysiological dose of OT, respectively. Eight cows were assigned to 2 treatments during 2 milkings according to a crossover experimental design: Control+OT (cows were first milked to collect standard milk and then received 5 IU of OT to collect residual milk through a second milking) and Ato + OT (cows were injected with Ato (50 μg/kg of body weight) and milked to collect cisternal milk, then received 5 IU of OT to collect alveolar milk through a second milking). Milk MEC were purified to determine their concentration and number in milk. Mammary epithelium integrity was assessed by measuring the kinetics of plasma lactose concentration. Inhibiting myoepithelial cell contraction by Ato injection decreased the number of exfoliated MEC in milk. In contrast, OT injection increased the concentration of MEC in the residual milk and the number of MEC in the alveolar milk. Ato injection reduced plasma lactose concentration, whereas, in both treatments, OT injections increased it. Our results suggested that myoepithelial cell contraction caused by OT could stimulate MEC exfoliation into milk and was associated with epithelium disruption.
Databáze: OpenAIRE
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