Determinação de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extração sequencial

Autor:	Rodinei Augusti, Agenor Valadares Santos, Leila M. Bittencourt, Ana Paula F. Gonçalves, Letícia M. Costa, Adriano Marçal Pimenta, Diogo A. P. D. Lana
Jazyk:	portugalština
Rok vydání:	2012
Předmět:	Globulin biology Plant composition Extraction (chemistry) chemistry.chemical_element metals General Chemistry Manganese Zinc Protein content lcsh:Chemistry chemistry lcsh:QD1-999 Botany biology.protein sequential extraction protein Chemical composition Total protein Nuclear chemistry
Zdroj:	Química Nova v.35 n.10 2012 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ Química Nova, Vol 35, Iss 10, Pp 1922-1926 (2012)
Popis:	A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L-1 NaCl, ethanol/water (70:30 v v-1) and 0.5 mol L-1 NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method.
Databáze:	OpenAIRE
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