Transposon Mutagenesis Reveals Differential Pathogenesis of Ralstonia solanacearum on Tomato and Arabidopsis
| Autor: | Fang-I Ho, Shu-Mei Huang, Der-Kang Lu, Hwei-Ling Shen, Mounira Elbaz, Jaw-Fen Wang, Pei-Cheng Huang, Yu-Mei Lin, I-Chun Chou, Chiu-Ping Cheng, Yu-Ju Chu |
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| Rok vydání: | 2008 |
| Předmět: |
Transposable element
Virulence Factors Physiology Arabidopsis Mutagenesis (molecular biology technique) Virulence Solanum lycopersicum Botany Arabidopsis thaliana Gene Genetics Ralstonia solanacearum Models Genetic biology fungi food and beverages General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Mutagenesis Insertional Genes Bacterial Mutation DNA Transposable Elements Transposon mutagenesis Agronomy and Crop Science |
| Zdroj: | Molecular Plant-Microbe Interactions®. 21:1261-1270 |
| ISSN: | 1943-7706 0894-0282 |
| Popis: | Ralstonia solanacearum causes a deadly wilting disease on a wide range of crops. To elucidate pathogenesis of this bacterium in different host plants, we set out to identify R. solanacearum genes involved in pathogenesis by screening random transposon insertion mutants of a highly virulent strain, Pss190, on tomato and Arabidopsis thaliana. Mutants exhibiting various decreased virulence levels on these two hosts were identified. Sequence analysis showed that most, but not all, of the identified pathogenesis genes are conserved among distinct R. solanacearum strains. A few of the disrupted loci were not reported previously as being involved in R. solanacearum pathogenesis. Notably, a group of mutants exhibited differential pathogenesis on tomato and Arabidopsis. These results were confirmed by characterizing allelic mutants in one other R. solanacearum strain of the same phylotype. The significantly decreased mutants' colonization in Arabidopsis was found to be correlated with differential pathogenesis on these two plants. Differential requirement of virulence genes suggests adaptation of this bacterium in different host environments. Together, this study reveals commonalities and differences of R. solanacearum pathogenesis on single solanaceous and nonsolanaceous hosts, and provides important new insights into interactions between R. solanacearum and different host plants. |
| Databáze: | OpenAIRE |
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