Simultaneous analyte indicator binding assay (SBA) for the monitoring of reversible host���guest complexation kinetics
| Autor: | Zsombor Miskolczy, Stephan Sinn, Frank Biedermann, László Biczók, Mónika Megyesi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Analyte
Technology Chemistry Ligand binding assay Dissociation rate Kinetics Complex formation Metals and Alloys Rational design General Chemistry macromolecular substances Catalysis Dissociation (chemistry) Surfaces Coatings and Films Electronic Optical and Magnetic Materials Computational chemistry Materials Chemistry Ceramics and Composites ddc:600 Binding affinities |
| Zdroj: | Chemical communications, 57 (94), 12663–12666 |
| ISSN: | 1359-7345 1364-548X |
| DOI: | 10.5445/ir/1000141667 |
| Popis: | Very little information is available on the kinetics of the self-assembly and dissociation of optically silent building blocks despite the importance of such data in the rational design of tailor-made host���guest systems. We introduce here a novel time-resolved method that enables the simultaneous determination of complex formation and complex dissociation rate constants for inclusion-type host���guest complexes. The simultaneous analyte indicator binding assay (SBA) gives also direct access to binding affinities, thus largely simplifying the experimental procedure for a full kinetic and thermodynamic characterisation of host���guest systems. |
| Databáze: | OpenAIRE |
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