Spatial and temporal expression of CCR3 and the common beta chain of the IL-3, IL-5 and GM-CSF receptor in the nasal epithelium and lymphoid tissues in a rat model of allergic rhinitis
| Autor: | Paolo M. Renzi, James G. Wagner, Nicolay Ferrari, Julie Brault, Marylène Fortin, Jack R. Harkema, Luc Paquet |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Lymphoid Tissue Receptors CCR3 Immunology CCR3 Mucous membrane of nose medicine.disease_cause Polymerase Chain Reaction Biochemistry Hypersensitivity medicine Animals Immunology and Allergy RNA Messenger Receptor Molecular Biology Interleukin 5 DNA Primers Rhinitis Base Sequence biology GM-CSF Receptor Hematology Rats Disease Models Animal Nasal Mucosa Ovalbumin Receptors Granulocyte-Macrophage Colony-Stimulating Factor Allergic response biology.protein Interleukin-3 Interleukin-5 CC chemokine receptors |
| Zdroj: | Cytokine. 52:194-202 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2010.07.005 |
| Popis: | Background Allergic rhinitis (AR) and asthma are closely related conditions that often co-exist, and are characterized by a Th2 inflammatory response where eosinophils occupy a predominant role. Strategies aimed at blocking signaling through the CC chemokine receptor 3 (CCR3) and/or the common beta chain of the IL-3, IL-5 and GM-CSF receptor (βc) efficiently reduced eosinophilic inflammation in both animal models and in asthmatic patients. This study was therefore aimed at characterizing the spatio-temporal expression pattern of βc and CCR3 using a rat model of AR. Methods Sensitized rats were challenged with ovalbumin and sacrificed at 2 h, 8 h, 16 h or 24 h post-challenge. Nasal tissues were microdissected and used for mRNA quantification by QPCR, while histological evaluation determined the presence of eosinophils and mucosubstances. Results Allergen-induced recruitment of eosinophils in the distal septum and turbinates was maximal at 8 h post-challenge, and was correlated with 2–4-fold increase in CCR3 and βc mRNA. Recruitment of eosinophils was also accompanied by upregulated IL-5, IL-4Rα, TNF-α and IFN-γ mRNA at early time-points. In contrast, IL-13 and MUC5AC mRNA, as well as production of mucosubstances were maximal at 24 h. Conclusions βc and CCR3 could play important roles in the modulation of the allergic response, and their inhibition may represent a promising therapeutic approach for AR. |
| Databáze: | OpenAIRE |
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