Involvement of granzyme B and granulysin in the cytotoxic response in lichen planus

Autor: Mourad Mokni, Mohammed Ammar, A. Ben Osman, Amel El Gaied, Samir Boubaker, Hechmi Louzir
Přispěvatelé: Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Laboratoire d'immunologie, de génétique et de pathologie humaine, Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM)-Université de Tunis El Manar (UTM), Hôpital La Rabta [Tunis]
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Antigens
Differentiation
T-Lymphocyte
Male
Pathology
Apoptosis
Granzymes
030207 dermatology & venereal diseases
0302 clinical medicine
Immunophenotyping
MESH: Reverse Transcriptase Polymerase Chain Reaction
MESH: Child
Cytotoxic T cell
Granulysin
Cytotoxicity
Child
skin and connective tissue diseases
MESH: Antigens
CD
Skin
MESH: Statistics
Nonparametric
MESH: Middle Aged
biology
integumentary system
Reverse Transcriptase Polymerase Chain Reaction
Lichen Planus
Middle Aged
Immunohistochemistry
3. Good health
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
030220 oncology & carcinogenesis
Female
Adult
medicine.medical_specialty
Histology
Adolescent
MESH: Immunophenotyping
Dermatology
Statistics
Nonparametric
Pathology and Forensic Medicine
MESH: Lichen Planus
03 medical and health sciences
Antigen
stomatognathic system
MESH: Skin
Antigens
CD
medicine
Humans
RNA
Messenger
MESH: RNA
Messenger
MESH: Adolescent
MESH: Granzymes
MESH: Humans
MESH: Apoptosis
MESH: Adult
MESH: Immunohistochemistry
MESH: Male
Granzyme B
stomatognathic diseases
Granzyme
MESH: Antigens
Differentiation
T-Lymphocyte
Immunology
biology.protein
MESH: T-Lymphocytes
Cytotoxic
MESH: Female
CD8
T-Lymphocytes
Cytotoxic
Zdroj: Journal of Cutaneous Pathology
Journal of Cutaneous Pathology, Wiley, 2008, 35 (7), pp.630-4. ⟨10.1111/j.1600-0560.2007.00892.x⟩
ISSN: 0303-6987
1600-0560
DOI: 10.1111/j.1600-0560.2007.00892.x⟩
Popis: International audience; BACKGROUND: Lichen planus is an inflammatory dermatosis involving either skin and/or mucosal epithelial surfaces. A cell-mediated cytotoxicity response is the main suspected mechanism of this dermatosis. Granzyme B and granulysin are components of the cytoplasmic granules of cytotoxic T lymphocytes and natural killers. They are involved in cell-mediated apoptosis. This work studies the possible implication of granzyme B and granulysin in the cell-mediated cytotoxicity response in lichen planus. METHODS: In situ expression of granzyme B and granulysin was studied by real-time reverse transcriptase polymerase chain reaction in 15 biopsies of lichen planus. The distribution and the phenotype of the inflammatory infiltrate and the expression of granzyme B were studied by immunohistochemistry in seven other biopsies of lichen planus. RESULTS: Granzyme B and granulysin mRNA expression was one to two hundred times greater than in biopsies of normal skin. Immunohistochemical study revealed that the lymphohistiocytic infiltrate consisted mainly of CD4+ and CD8+ lymphocytes. Granzyme B+ cells were observed close to apoptotic keratinocytes. CONCLUSION: Our results suggest a central role for cell-mediated cytotoxicity by the granule exocytosis pathway probably because of auto-cytotoxic T-cell clones in the pathogenesis of lichen planus.
Databáze: OpenAIRE
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