Anti-proliferative and cytotoxic activity of pentadactylin isolated from Leptodactylus labyrinthicus on melanoma cells
| Autor: | Graziella Anselmo Joanitti, Wagner Fontes, Eduardo Maffud Cilli, Mariana S. Castro, Osmindo Rodrigues Pires Júnior, Anna Christina C. Nascimento, Michelle S. Libério, Marcelo Valle de Sousa, Ricardo Bentes Azevedo, Lanuse C. Zanotta |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Clinical Biochemistry Antineoplastic Agents Apoptosis Biology Cell morphology Biochemistry 060199 Biochemistry and Cell Biology not elsewhere classified Mice Cell Line Tumor Cytotoxic T cell Animals Humans Secretion Viability assay Cytotoxicity Melanoma Cell Proliferation Skin B16F10 Cytotoxins Organic Chemistry Cell Cycle Amphibian Cell biology Leptodactylus labyrinthicus Cell culture Cancer cell cytotoxicity Anura 111204 Cancer Therapy (excl. Chemotherapy and Radiation Therapy) Antimicrobial Cationic Peptides |
| Zdroj: | Amino Acids |
| ISSN: | 1438-2199 |
| Popis: | Nowadays, the emergence of resistance to the current available chemotherapeutic drugs by cancer cells makes the development of new agents imperative. The skin secretion of amphibians is a natural rich source of antimicrobial peptides (AMP), and researchers have shown that some of these wide spectrum molecules are also toxic to cancer cells. The aim of this study was to verify a putative anticancer activity of the AMP pentadactylin isolated for the first time from the skin secretion of the frog Leptodactylus labyrinthicus and also to study its cytotoxic mechanism to the murine melanoma cell line B16F10. The results have shown that pentadactylin reduces the cell viability of B16F10 cells in a dose-dependent manner. It was also cytotoxic to normal human fibroblast cells; nevertheless, pentadactylin was more potent in the first case. The studies of action mechanism revealed that pentadactylin causes cell morphology alterations (e.g., round shape and shrinkage morphology), membrane disruption, DNA fragmentation, cell cycle arrest at the S phase, and alteration of mitochondrial membrane potential, suggesting that B16F10 cells die by apoptosis. The exact mechanism that causes reduction of cell viability and cytotoxicity after treatment with pentadactylin is still unknown. In conclusion, as cancer cells become resilient to death, it is worthwhile the discovery of new drugs such as pentadactylin that induces apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink