Identification of in vitro differential cell secretions due to cigarette smoke condensate exposure using nanoflow capillary liquid chromatography and high-resolution mass spectrometry
| Autor: | Jason W. Flora, Timothy B. Langston, Willie J. McKinney, Gaurav S. J. B. Rana, Guoya Li, Rebecca Secrist, Jeff Edmiston |
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| Rok vydání: | 2008 |
| Předmět: |
Proteomics
Bodily Secretions Cell signaling Cell Survival Molecular Sequence Data Cell Biochemistry Mass Spectrometry Analytical Chemistry In vivo Smoke Tobacco medicine Humans Nanotechnology Amino Acid Sequence Inhalation Exposure Chromatography Chemistry Endothelial Cells In vitro Endothelial stem cell medicine.anatomical_structure Secretory protein Cell culture Apoptosis Peptides Chromatography Liquid |
| Zdroj: | Analytical and Bioanalytical Chemistry. 391:2845-2856 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-008-2197-3 |
| Popis: | Secreted proteins, the secretome, can be isolated from biological fluids (e.g., blood) and are often responsible for the regulation of biological processes such as cell signaling, growth, and apoptosis. The identification of secreted proteins can lead to an understanding of disease mechanisms and they can serve as early candidate biomarkers of disease and exposure. However, it is time-consuming and costly to conduct in vivo interrogations of the human secretome. The purpose of this article is to provide a detailed description of a rapid in vitro technique for the analysis of differential protein secretion due to exposure to smoking-machine-generated cigarette smoke (CS) condensate (total particulate matter, TPM). Endothelial cells were exposed to CS-TPM, the supernatant was collected, and the secretome was elucidated by nano liquid chromatography coupled with high-resolution mass spectrometry. A total of 1,677 unique peptides were identified in the cell culture supernatants. Several proteins were differentially expressed following CS-TPM exposure that relate to several biological processes, such as metabolism, development, communication, response to stimulus, and response to stress. |
| Databáze: | OpenAIRE |
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