Increased Expression of Major Basic Protein (MBP) and Interleukin‐5 (IL‐5) in Middle Ear Biopsy Specimens from Atopic Patients with Persistent Otitis Media with Effusion
| Autor: | David S. Hurst, Erin D. Wright, Deborah Miotto, Chantal Giguere, Qutayba Hamid |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Allergy
CD3 Complex Biopsy Ear Middle Enzyme-Linked Immunosorbent Assay Allergic inflammation 03 medical and health sciences Ribonucleases 0302 clinical medicine otorhinolaryngologic diseases medicine Humans Child 030223 otorhinolaryngology Interleukin 5 In Situ Hybridization biology medicine.diagnostic_test Otitis Media with Effusion business.industry Blood Proteins Eosinophil Granule Proteins medicine.disease Immunohistochemistry medicine.anatomical_structure Otitis Otorhinolaryngology Effusion Child Preschool 030220 oncology & carcinogenesis Chronic Disease Immunology Middle ear Major basic protein biology.protein Surgery Inflammation Mediators Interleukin-5 medicine.symptom business |
| Zdroj: | Otolaryngology–Head and Neck Surgery. 123:533-538 |
| ISSN: | 1097-6817 0194-5998 |
| DOI: | 10.1067/mhn.2000.109472 |
| Popis: | Background: Molecular biologic evidence to support an etiologic role for allergy in the pathogenesis of persistent otitis media with effusion (OME) is lacking. Objective: The goal of this article was to document expression of allergy-associated Th-2–type cytokines and inflammatory cells in the middle ear mucosa of children with persistent OME. Methods: With immunocytochemistry (CD3, major basic protein) and in situ hybridization (interleukin-5 mRNA), middle ear biopsy specimens from 7 children with persistent OME were stained. Nonatopic stapedectomy patients with no history of otitis media served as controls (n = 7). Results: There was a statistically significant (P < 0.05) difference in expression of CD3, major basic protein, and interleukin-5 between experimental and control subjects. All 8 OME patients proved to be atopic by ELISA testing. Conclusions: Type I allergy involving a Th-2–type cytokine and cellular profile may be a contributing factor in the persistence of OME in atopic children. Significance: The middle ear may serve as a target organ for allergic inflammation, suggesting that appropriate allergy management may be a useful adjunct to the management of OME. (Otolaryngol Head Neck Surg 2000;123:533-8.) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text