Molecular cloning and functional characterization of the zebrafish ATP-gated ionotropic receptor P2X(3) subunit
| Autor: | Mark M. Voigt, Jane A. Cox, Terrance M. Egan |
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| Rok vydání: | 2000 |
| Předmět: |
Agonist
animal structures medicine.drug_class Protein subunit Purinoceptor Molecular Sequence Data Biophysics Danio Molecular cloning Biochemistry Gamma-aminobutyric acid receptor subunit alpha-1 Interleukin 10 receptor alpha subunit Structural Biology Genetics medicine Animals Transmitter-gated channel Amino Acid Sequence Cloning Molecular Receptor Molecular Biology Zebrafish biology Receptors Purinergic P2 Cell Biology biology.organism_classification Molecular biology Cell biology Rats body regions Nucleotide Sequence Alignment |
| Zdroj: | FEBS letters. 475(3) |
| ISSN: | 0014-5793 |
| Popis: | We describe a P2X subunit cloned from the zebrafish (Danio rerio) that is an orthologue of the mammalian P2X(3) subunit. Like the mammalian P2X(3), this receptor desensitizes rapidly in the presence of agonist. However, it differs in that alphabeta-meATP is a much less potent agonist than ATP and the antagonist TNP-ATP is not active at low nanomolar concentrations. Similar to the rat P2X(3) subunit, the zebrafish subunit forms hetero-oligomeric assemblies with the rat P2X(2) that possesses a phenotype distinct from either parent. This novel clone will provide an important basis for future experiments investigating the structure/function relationships of P2X subunit domains. |
| Databáze: | OpenAIRE |
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