Effects ofCordyceps sinensison the Expressions of NF-κB and TGF-β1 in Myocardium of Diabetic Rats

Autor: Hua Gao, You-you Gu, Su Wang, Ming-cai Qiu, Huan Wang
Rok vydání: 2015
Předmět:
Zdroj: Evidence-based Complementary and Alternative Medicine : eCAM
Evidence-Based Complementary and Alternative Medicine, Vol 2015 (2015)
ISSN: 1741-4288
1741-427X
Popis: Objective.To investigate the effect ofCordyceps sinensis(CS) on the expressions of NF-κB and TGF-β1 in myocardium of streptozotocin-induced diabetic rats.Methods.A total of 53 healthy male SD rats, mice age of 8 weeks and weight of 220 ± 20 g, were randomly divided into five groups by randomized block design: normal control group (n=10), diabetic group (n=10), low dose of CS group (n=12; CS 0.6 g·kg−1·d−1), middle dose of CS group (n=11; CS 2.5 g·kg−1·d−1), and high dose of CS group (n=10; CS 5 g·kg−1·d−1). The diabetic models with tail intravenous injection by streptozotocin (45 mg·kg−1). Diabetic rats were sacrificed after 8 weeks; the expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle were determined by using immunohistochemistry staining and reverse transcription polymerase chain reaction (RT-PCR) method. The data were analyzed using one factor analysis of variance.Result.The expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle of diabetic rats were significantly raised (P<0.05), which could be decreased by CS (P<0.05).Conclusions.The changes on the expressions of NF-κB and TGF-β1 in myocardium may be involved in the occurrence of diabetic cardiomyopathy (DC). CS may play its role on myocardial protection by regulating the expressions of NF-κB and TGF-β1 in myocardium.
Databáze: OpenAIRE
Externí odkaz: