Tumor necrosis factor α and interferon γ genes polymorphisms and serum levels in pancreatic adenocarcinoma
| Autor: | B. Smolarz, Gasiorowska A, A. Kulig, Renata Talar-Wojnarowska, Romanowicz-Makowska H, E Malecka-Panas |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Cachexia CD30 Adenocarcinoma Polymerase Chain Reaction Polymorphism Single Nucleotide Gastroenterology Interferon-gamma Internal medicine parasitic diseases Genotype Humans Medicine Genetic Predisposition to Disease Clinical significance Interferon gamma Aged Aged 80 and over Tumor Necrosis Factor-alpha business.industry Middle Aged medicine.disease Pancreatic Neoplasms Pancreatitis Oncology Cancer research Female Tumor necrosis factor alpha business Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Neoplasma. 56:56-62 |
| ISSN: | 0028-2685 |
| DOI: | 10.4149/neo_2009_01_56 |
| Popis: | Several biochemical pathways can lead to cancer cachexia, one of which involves the elevation of the cytokines, such as tumor necrosis factor alpha (TNF-alpha) and interferon gamma (INF-gamma). It was suggested that TNF-alpha and INF-gamma genes polymorphisms may influence these cytokines serum levels, but published data are still controversial. The aim of our study was to assess the clinical significance of -308G/A TNF-alpha and +874A/T INF-gamma genes polymorphisms as well as TNF-alpha and INF-gamma serum levels in pancreatic adenocarcinoma (PA) and chronic pancreatitis (CP) as regards to healthy volunteers. We studied 41 patients with pancreatic adenocarcinoma, 56 with chronic pancreatitis and 50 healthy volunteers. Peripheral venous blood samples were obtained from all patients for TNF-alpha and INF-gamma serum concentrations measurement. After DNA isolation TNF-alpha and INF-gamma genes polymorphisms have been studied using restriction fragment length polymorphism (RFLP) analysis. Plasma levels of TNF-alpha were significantly higher in PA patients (32.7 pg/ml) compared with CP patients (3.2 pg/ ml) and control group ( |
| Databáze: | OpenAIRE |
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