Adrenergic signalling between rat taste receptor cells
| Autor: | Tiansheng Shen, Shao-gang Lu, Scott Herness, Xiao‐Dong Sun, Fang-li Zhao, Namik Kaya |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Beta-3 adrenergic receptor
medicine.medical_specialty Physiology Gene Expression Adrenergic Alpha-1B adrenergic receptor Synaptic Transmission Membrane Potentials Rats Sprague-Dawley Beta-1 adrenergic receptor Norepinephrine Taste receptor Internal medicine Paracrine Communication Receptors Adrenergic beta Taste bud medicine Animals Neurons Afferent RNA Messenger Potassium Channels Inwardly Rectifying Alpha-1D adrenergic receptor Chemistry Isoproterenol Epithelial Cells Original Articles Adrenergic beta-Agonists Receptors Adrenergic alpha Taste Buds Immunohistochemistry Alpha-1A adrenergic receptor Rats medicine.anatomical_structure Endocrinology Taste Potassium Adrenergic alpha-Agonists |
| Zdroj: | The Journal of Physiology. 543:601-614 |
| ISSN: | 1469-7793 0022-3751 |
| DOI: | 10.1113/jphysiol.2002.020438 |
| Popis: | In taste buds, synaptic transmission is traditionally thought to occur from taste receptor cells to the afferent nerve. This communication reports the novel observation that taste receptor cells respond to adrenergic stimulation. Noradrenaline application inhibited outward potassium currents in a dose-dependent manner. This inhibition was mimicked by the beta agonist isoproterenol and blocked by the beta antagonist propranolol. The alpha agonists clonidine and phenylephrine both inhibited the potassium currents and elevated intracellular calcium levels. Inwardly rectifying potassium currents were unaffected by adrenergic stimulation. Experiments using the RT-PCR technique demonstrate that lingual epithelium expresses multiple alpha (alpha1a, alpha1b, alpha1c, alpha1d, alpha2a, alpha2b, alpha2c) and beta (beta1, beta2) subtypes of adrenergic receptors, and immunocytochemistry localized noradrenaline to a subset of taste receptor cells. Collectively, these data imply strongly that adrenergic transmission within the taste bud may play a paracrine role in taste physiology. |
| Databáze: | OpenAIRE |
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