Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens
| Autor: | Asep Gunawan, T. Sartika, Cece Sumantri, M. Ardiyana, Sri Murtini |
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| Rok vydání: | 2020 |
| Předmět: |
Disease resistance
iNOS gene General Veterinary biology Salmonella enteritidis Antibody titer Immunogenetics NRAMP-1 gene biology.organism_classification Newcastle disease polymorphism Microbiology SenSi-1 Agrinak chicken Immune system Genotype biology.protein Immunoglobulin Y Animal Science and Zoology lcsh:Animal culture Antibody lcsh:SF1-1100 |
| Zdroj: | Tropical Animal Science Journal, Vol 43, Iss 2 (2020) |
| ISSN: | 2615-790X 2615-787X |
| DOI: | 10.5398/tasj.2020.43.2.95 |
| Popis: | NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens. |
| Databáze: | OpenAIRE |
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