Characterization of isolates of Eisenbergiella tayi , a strictly anaerobic Gram-stain variable bacillus recovered from human clinical materials in Canada
| Autor: | Tamara Burdz, Tina Zimmerman, Linda Hoang, Deborah Wiebe, Anne-Marie Bernier, Kathryn Bernard, Brittany M. Balcewich, Philippe Lagacé-Wiens |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
DNA Bacterial Male 0301 basic medicine Canada Adolescent 030106 microbiology Bacteremia DNA Ribosomal Microbiology Genome law.invention Young Adult 03 medical and health sciences Phylogenetics law RNA Ribosomal 16S medicine Cluster Analysis Humans Blood culture Gram-Positive Bacterial Infections Phylogeny Aged Aged 80 and over Genetics Bacillus (shape) Whole genome sequencing Bacteriological Techniques Base Composition Clostridiales biology medicine.diagnostic_test Nucleic Acid Hybridization Sequence Analysis DNA Middle Aged Appendicitis biology.organism_classification 030104 developmental biology Infectious Diseases Gram staining Eisenbergiella tayi Female Genome Bacterial |
| Zdroj: | Anaerobe. 44:128-132 |
| ISSN: | 1075-9964 |
| DOI: | 10.1016/j.anaerobe.2017.03.005 |
| Popis: | Eisenbergiella gen. nov. was proposed in 2014 to describe an obligate anaerobic, structurally Gram-positive but Gram-stain-negative-appearing bacillus recovered from the blood culture of an elderly Israeli man. Here, we describe features for eight blood culture isolates as well one appendix-derived isolate, recovered from seven patients located in two Canadian provinces, which by 16S rRNA gene sequencing, were identifiable as Eisenbergiella tayi, the sole validly- named species in this genus. After whole genome sequencing, isolates were found to be essentially identical (96.8-98.7% identity) to each other and to E. tayi DSM 26961T, after comparison using the ANIb tool and in silico DNA-DNA hydridization. All isolates were observed to have remarkably large genomes (7.1-8.3 Mb) with a G + C content of 46.5%-46.9%. |
| Databáze: | OpenAIRE |
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