Characterization of liver X receptor expression and function in human skin and the pilosebaceous unit
| Autor: | Christos C. Zouboulis, Jacky M. Burrin, Wesley J. Harrison, Adiam W. Bahta, Louise E. Russell, Michael P. Philpott |
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| Rok vydání: | 2007 |
| Předmět: |
Keratinocytes
medicine.medical_specialty Blotting Western Gene Expression Receptors Cytoplasmic and Nuclear Human skin Dermatology Biology Biochemistry Cell Line Sebaceous Glands Internal medicine medicine Humans Receptor Liver X receptor Molecular Biology Cells Cultured Cell Proliferation Liver X Receptors Phenylacetates Skin Sulfonamides integumentary system Cell growth Reverse Transcriptase Polymerase Chain Reaction Lipogenesis Liver X receptor alpha Fibroblasts Hair follicle Orphan Nuclear Receptors Cell biology Sweat Glands DNA-Binding Proteins Endocrinology medicine.anatomical_structure Nuclear receptor Epidermal Cells Cell culture Epidermis Hair Follicle Hair |
| Zdroj: | Experimental dermatology. 16(10) |
| ISSN: | 0906-6705 |
| Popis: | The nuclear receptors liver X receptor alpha (LXRalpha) and liver X-receptor beta (LXRbeta) have a well documented role in cholesterol homeostasis and lipid metabolism within tissues and cells including the liver, small intestine and macrophages. In keratinocytes, LXRs have been shown to up-regulate differentiation in vitro via increased transcription of proteins of the AP1 complex and to down-regulate proliferation in vivo. In this study, we provide a detailed description of the location and possible role of LXRs within human skin and its associated glands and appendages. Using RT-PCR, Western blotting and immunohistochemistry, we have demonstrated expression of LXRalpha and LXRbeta mRNA and proteins in whole human skin as well as within a range of primary and immortalized human cell lines derived from human skin, hair follicle and sebaceous glands. Furthermore, we have shown that synthetic LXR specific agonists GW683965 and TO901317 significantly inhibit cell proliferation in primary epidermal keratinocytes, immortalized N/TERT keratinocytes and the immortalized SZ95 sebocyte line, and significantly increase lipogenesis in SZ95 sebocytes. In addition, we showed that the synthetic agonist TO901317 significantly reduced hair growth, in vitro. |
| Databáze: | OpenAIRE |
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