Association betweenFokI,ApaIandTaqIRFLP polymorphisms in VDR gene and Hashimoto's thyroiditis: preliminary data from female patients in Serbia

Autor:	K. Savic, Öztürk Özdemir, Jelena Djurovic, Cisem Akurut, Oliver Stojkovic, Gorana Stamenković, Jelena Todorovic, Fatma Silan
Přispěvatelé:	[Djurovic, J. -- Stojkovic, O. -- Savic, K.] Univ Belgrade, Inst Forens Med, Fac Med, Belgrade, Serbia -- [Ozdemir, O.] Cumhuriyet Univ, Dept Med Genet, Fac Med, Sivas, Turkey -- [Ozdemir, O. -- Silan, F. -- Akurut, C.] Canakkale Onsekiz Mart Univ, Dept Med Genet, Fac Med, Canakkale, Turkey -- [Stamenkovic, G.] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade, Serbia, Stojkovic, Oliver -- 0000-0002-4794-1882, Stamenkovic, Gorana -- 0000-0002-5809-7189
Rok vydání:	2015
Předmět:	medicine.medical_specialty Genotype TaqI Immunology Population Hashimoto Disease Biology Calcitriol receptor Thyroiditis chemistry.chemical_compound Sex Factors Gene Frequency Internal medicine Odds Ratio Genetics medicine Humans Genetic Predisposition to Disease education Molecular Biology Alleles Genetic Association Studies Genetics (clinical) education.field_of_study Thyroid General Medicine medicine.disease Thyroid disorder FokI 3. Good health medicine.anatomical_structure Endocrinology chemistry biology.protein Receptors Calcitriol Female Gene polymorphism Serbia Polymorphism Restriction Fragment Length
Zdroj:	International Journal of Immunogenetics
ISSN:	1744-3121
Popis:	WOS: 000354388300008 PubMed ID: 25817800 Hashimoto's thyroiditis (HT) is the most prevalent autoimmune thyroid disorder caused by an interaction between genes and environmental triggers. Intrathyroid lymphocytic infiltration may lead to progressive destruction of thyroid tissue and consequently to hypothyroidism. Many studies in different populations have shown association between vitamin D receptor (VDR) gene polymorphisms and various autoimmune diseases, including HT. The study included 44 female patients (mean age +/- standard deviation 38 +/- 5.4) with Hashimoto's thyroiditis and 32 healthy age-matched, sex-matched and geographically matched controls without personal history of autoimmune and endocrine diseases. Genomic DNA was isolated from peripheral blood-EDTA, and the target VDR gene was genotyped by PCR-RFLP technique after VDR-FokI (rs2228570), VDR-ApaI (rs7975232) and VDR-TaqI (rs731236) restriction enzymes digestion. We used spss 20.0 integrated software for data analysis and found a significant difference in the genotype distribution of VDR-FokI polymorphism between patients with HT and controls (P=0.009). For ApaI and TaqI, we observed a higher frequency of variant allele in patients with HT, which was not significantly different compared to control women (P>0.05). The current first and preliminary results identified the association between VDR-FokI gene polymorphism and Hashimoto's thyroiditis in Serbian population. Results need to be supported by further investigations that define haplotype patterns for VDR gene polymorphisms in a larger group of HT patients of both sexes. Serbian Ministry of Education, Science and Technology [OI 175093] This work was supported by the Serbian Ministry of Education, Science and Technology (Grant OI 175093).
Databáze:	OpenAIRE
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