Transcriptional inhibition of feline immunodeficiency virus by alpha-amanitin
| Autor: | Takashige Kashimoto, Kazuhiko Kawashima, Satomi Yamamoto, Yurina Fukuda, Hisaaki Sato, Taishi Tanabe |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Amanita
endocrine system Feline immunodeficiency virus animal structures 040301 veterinary sciences animal diseases viruses alpha-Amanitin Immunodeficiency Virus Feline Antiviral Agents 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Transcription (biology) Virology feline immunodeficiency virus (FIV) polycyclic compounds Animals Alpha-Amanitin 030304 developmental biology Amanitin chemistry.chemical_classification therapy 0303 health sciences amanitin General Veterinary biology Chemistry anti-retroviral drug 04 agricultural and veterinary sciences Note biology.organism_classification Cyclic peptide Reverse transcriptase Blot Cats |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Alpha-amanitin, one of the amatoxins in egg amanita, has a cyclic peptide structure, and was reported as having antiviral activity against several viruses. We investigated whether α-amanitin has antiviral activity against feline immunodeficiency virus (FIV). FL-4 cells persistently infected with FIV Petaluma were cultured with α-amanitin. Reverse transcriptase (RT) activity in the supernatant of FL-4 cells was significantly inhibited by α-amanitin. In addition, the production of FIV core protein in FL-4 cells was inhibited by α-amanitin when analyzed by western blotting. Furthermore, α-amanitin inhibited the transcription of FIV in real-time RT-PCR. These data suggested that α-amanitin showed anti-FIV activity by inhibiting the RNA transcription level. |
| Databáze: | OpenAIRE |
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