Liquid chromatographic separation of the enantiomers of trans-chlordane, cis-chlordane, heptachlor, heptachlor epoxide and α-hexachlorocyclohexane with application to small-scale preparative separation
| Autor: | Jennifer C DiMarco, William L Champion, James Lee, Aki Matabe, Keith B. Prickett, A. Wayne Garrison |
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| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Heptachlor Epoxide Heptachlor Metabolite Organic Chemistry Chlordane General Medicine alpha-Hexachlorocyclohexane Biochemistry High-performance liquid chromatography Analytical Chemistry Hexane chemistry.chemical_compound Isomerism chemistry Chlordan Organic chemistry Enantiomer Chromatography High Pressure Liquid Hexachlorocyclohexane |
| Zdroj: | Journal of Chromatography A. 1024:55-62 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2003.10.026 |
| Popis: | Analytical high-performance liquid chromatographic separations of the individual enantiomers of five polychlorinated compounds were obtained on polysaccharide stereoselective HPLC columns. The enantiomers of the pesticides trans-chlordane, cis-chlordane and heptachlor were separated on CHIRALCEL OD using a hexane mobile phase. The enantiomers of the heptachlor metabolite, heptachlor epoxide, were separated on CHIRALPAK AD using a methanol mobile phase. The enantiomers of alpha-hexachlorocyclohexane (alpha-HCH), were separated on CHIRALCEL OJ using a hexane/2-propanol mobile phase. Similar chromatographic conditions using preparative columns were used to isolate approximately 250 mg of each of the individual enantiomers. The purified individual enantiomers have been submitted for testing of their endocrine disruptor (ED) activity. |
| Databáze: | OpenAIRE |
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