Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center
| Autor: | Gabriela Ibáñez-Cervantes, Juan Carlos Bravata-Alcántara, Juan Manuel Bello-López, Oscar Sosa-Hernández, Emilio Mariano Durán-Manuel, Clemente Cruz-Cruz, Rocío Flores-Paz, Julio Cesar Juárez-Gómez, Iliana Alejandra Cortés-Ortíz, Víctor Hugo Gutiérrez-Muñoz, Noé Escobar-Escamilla, Laura Delgado-Balbuena, Concepción Cu-Quijano |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Klebsiella pneumoniae 030106 microbiology Microbial Sensitivity Tests Integron Disease cluster Microbiology beta-Lactamases Disease Outbreaks Integrons law.invention 03 medical and health sciences 0302 clinical medicine law Drug Resistance Multiple Bacterial Virology Epidemiology medicine Humans 030212 general & internal medicine Typing Mexico Aged Aged 80 and over Cross Infection biology Transmission (medicine) Outbreak General Medicine Middle Aged biology.organism_classification Intensive care unit Hospitals Anti-Bacterial Agents Klebsiella Infections Infectious Diseases biology.protein Female Parasitology |
| Zdroj: | The Journal of Infection in Developing Countries. 15:657-664 |
| ISSN: | 1972-2680 |
| Popis: | Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak. Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed. Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”. Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel. |
| Databáze: | OpenAIRE |
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